This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-27_S67_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-27_S67_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.23 s (10 µs/read; 5.86 M reads/minute). === Summary === Total reads processed: 22,845 Reads with adapters: 221 (1.0%) Reads written (passing filters): 22,845 (100.0%) Total basepairs processed: 6,853,500 bp Total written (filtered): 6,852,433 bp (100.0%) === Adapter 1 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 221 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 80 357.0 0 80 4 119 89.2 0 119 15 1 0.0 1 0 1 16 21 0.0 1 1 20