This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-2_S42_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-2_S42_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.21 s (9 µs/read; 6.36 M reads/minute). === Summary === Total reads processed: 22,466 Reads with adapters: 359 (1.6%) Reads written (passing filters): 22,466 (100.0%) Total basepairs processed: 6,739,800 bp Total written (filtered): 6,738,488 bp (100.0%) === Adapter 1 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 359 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 279 351.0 0 279 4 67 87.8 0 67 15 1 0.0 1 0 1 16 12 0.0 1 2 10