This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-35_S75_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-35_S75_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.25 s (7 µs/read; 8.07 M reads/minute). === Summary === Total reads processed: 34,018 Reads with adapters: 488 (1.4%) Reads written (passing filters): 34,018 (100.0%) Total basepairs processed: 10,205,400 bp Total written (filtered): 10,203,827 bp (100.0%) === Adapter 1 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 488 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 380 531.5 0 380 4 107 132.9 0 107 5 1 33.2 0 1