This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-3_S43_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-3_S43_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.22 s (11 µs/read; 5.60 M reads/minute). === Summary === Total reads processed: 20,479 Reads with adapters: 138 (0.7%) Reads written (passing filters): 20,479 (100.0%) Total basepairs processed: 6,143,700 bp Total written (filtered): 6,143,102 bp (100.0%) === Adapter 1 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 138 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 122 320.0 0 122 4 2 80.0 0 2 16 14 0.0 1 0 14