This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -a AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-13_S53_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-13_S53_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.30 s (15 µs/read; 3.93 M reads/minute). === Summary === Total reads processed: 19,795 Reads with adapters: 155 (0.8%) Reads written (passing filters): 19,795 (100.0%) Total basepairs processed: 5,938,500 bp Total written (filtered): 5,937,761 bp (100.0%) === Adapter 1 === Sequence: AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC; Type: regular 3'; Length: 54; Trimmed: 5 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-54 bp: 5 Bases preceding removed adapters: A: 0.0% C: 20.0% G: 20.0% T: 60.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 309.3 0 4 4 1 77.3 0 1 === Adapter 2 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 150 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 81 309.3 0 81 4 52 77.3 0 52 16 17 0.0 1 6 11