This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -a AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-27_S67_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-27_S67_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.32 s (14 µs/read; 4.32 M reads/minute). === Summary === Total reads processed: 22,845 Reads with adapters: 230 (1.0%) Reads written (passing filters): 22,845 (100.0%) Total basepairs processed: 6,853,500 bp Total written (filtered): 6,852,399 bp (100.0%) === Adapter 1 === Sequence: AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC; Type: regular 3'; Length: 54; Trimmed: 10 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-54 bp: 5 Bases preceding removed adapters: A: 20.0% C: 40.0% G: 20.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 357.0 0 4 4 5 89.2 0 5 5 1 22.3 0 1 === Adapter 2 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 220 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 79 357.0 0 79 4 119 89.2 0 119 15 1 0.0 1 0 1 16 21 0.0 1 1 20