This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -a AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-5_S45_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-5_S45_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.32 s (12 µs/read; 4.82 M reads/minute). === Summary === Total reads processed: 25,342 Reads with adapters: 113 (0.4%) Reads written (passing filters): 25,342 (100.0%) Total basepairs processed: 7,602,600 bp Total written (filtered): 7,602,211 bp (100.0%) === Adapter 1 === Sequence: AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC; Type: regular 3'; Length: 54; Trimmed: 3 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-54 bp: 5 Bases preceding removed adapters: A: 33.3% C: 0.0% G: 33.3% T: 33.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 396.0 0 1 4 2 99.0 0 2 === Adapter 2 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 110 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 98 396.0 0 98 7 12 1.5 0 12