This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -a AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-NTC1_S79_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-NTC1_S79_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.71 s (9 µs/read; 7.00 M reads/minute). === Summary === Total reads processed: 83,413 Reads with adapters: 612 (0.7%) Reads written (passing filters): 83,413 (100.0%) Total basepairs processed: 25,023,900 bp Total written (filtered): 25,021,989 bp (100.0%) === Adapter 1 === Sequence: AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC; Type: regular 3'; Length: 54; Trimmed: 14 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-54 bp: 5 Bases preceding removed adapters: A: 7.1% C: 35.7% G: 28.6% T: 28.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 1303.3 0 4 4 7 325.8 0 7 5 2 81.5 0 2 8 1 1.3 0 1 === Adapter 2 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 598 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 539 1303.3 0 539 4 59 325.8 0 59