This is cutadapt 4.1 with Python 3.8.13 Command line parameters: --cores 16 --match-read-wildcards --overlap 3 --error-rate 0.1 -a AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC -g ACCTGCGGARGGATCA -o /nephele_data/outputs/cutadapt_trimmed/E23-ITS-NTC2_S80_L001_R1_001.fastq.gz /nephele_data/inputs/E23-ITS-NTC2_S80_L001_R1_001.fastq Processing single-end reads on 16 cores ... Finished in 0.46 s (11 µs/read; 5.43 M reads/minute). === Summary === Total reads processed: 41,264 Reads with adapters: 149 (0.4%) Reads written (passing filters): 41,264 (100.0%) Total basepairs processed: 12,379,200 bp Total written (filtered): 12,378,716 bp (100.0%) === Adapter 1 === Sequence: AACTTTYARCAAYGGATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC; Type: regular 3'; Length: 54; Trimmed: 6 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40-49 bp: 4; 50-54 bp: 5 Bases preceding removed adapters: A: 16.7% C: 33.3% G: 33.3% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 644.8 0 2 4 4 161.2 0 4 === Adapter 2 === Sequence: ACCTGCGGARGGATCA; Type: regular 5'; Length: 16; Trimmed: 143 times Minimum overlap: 3 No. of allowed errors: 1-9 bp: 0; 10-16 bp: 1 Overview of removed sequences length count expect max.err error counts 3 110 644.8 0 110 4 33 161.2 0 33