FastQCFastQC Report
Fri 31 Mar 2023
R22-all.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameR22-all.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839608
Sequences flagged as poor quality0
Sequence length300
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC257683.069051271545769No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT242482.8880144067231375No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC209222.4918771617230897No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC207252.4684138312164725No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC207112.4667463864088957No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT196202.336804794618441No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT189632.258553991862869No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC168372.0053405875122676No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC164131.9548408304827969No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT152931.8214452458766472No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC142461.6967441949100055No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC140491.6732808644033883No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT133251.5870501472115557No Hit
GTGTCAGCAGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC133201.5864546312088497No Hit
GTGTCAGCCGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC116771.39076807271965No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC116241.3844556030909663No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC116011.3817162294785186No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC114931.3688530838200683No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC110261.3132318891673256No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT110161.3120408571619135No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT108261.2894112490590848No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC95911.1423187963906967No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC94421.124572419510057No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCGAGCGTTGTCCGGATTTAC92731.1044439786185933No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT86351.0284561366733047No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC77520.9232880105954208No Hit
GTGCCAGCAGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC74430.8864851216281885No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCGAGCGTTGTCCGGATTTAC72780.8668330935388895No Hit
GTGCCAGCCGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC65160.7760764547264913No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC63120.7517794018160856No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC60890.7252193880953969No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT56620.6743623214643024No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATGCGAGCGTTGTCCGGATTTAC53270.6344627492829987No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT49250.5865832626654344No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC49140.5852731274594811No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT46090.5489466512944136No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT41300.4918962182351765No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCGAGCGTTGTCCGGATTTAC40200.4787948661756439No Hit
GTGTCAGCAGCCGCGGTAATACGAGGGGTGCAAGCGTTGTTCGGAATCAC39940.47569818296157257No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC33780.4023306114281903No Hit
GTGTCAGCCGCCGCGGTAATACGAGGGGTGCAAGCGTTGTTCGGAATCAC33480.3987575154119542No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT32700.3894674657697402No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC32210.3836314089432211No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT28340.3375384703337748No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT28100.33467999352078587No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC27810.33122600070509095No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC25430.30287943897628417No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC24700.2941849053367762No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC24200.28822974530971596No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC24100.2870387133043039No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT23970.28549037169726826No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTATCCGGATTTAC23590.28096445007670245No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT23020.27417556764585377No Hit
GTGCCAGCAGCCGCGGTAATACGAGGGGTGCAAGCGTTGTTCGGAATCAC22210.2645282084020162No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTTACAAGCGTTATCCGGATTTAC20850.2483301731284123No Hit
GTGTCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGAT20550.24475707711217615No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC20540.24463797391163494No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC20340.24225590990081086No Hit
GTGCCAGCCGCCGCGGTAATACGAGGGGTGCAAGCGTTGTTCGGAATCAC19970.23784909148078628No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC19790.23570523387104456No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC19700.23463330506617372No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGACAAGCGTTATCCGGATTTAC19690.23451420186563254No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC19300.22986917704452556No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT18720.22296119141313564No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC18570.22117464340501758No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTTACAAGCGTTATCCGGATTTAC18450.21974540499852313No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT18180.2165296185839106No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC18160.21629141218282816No Hit
GTGTCAGCAGCCGCGGTAACACGTAAGTTGCGAGCGTTGTTCGGAATTAT17590.20950252975197947No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC17460.20795418814494385No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC16180.1927089784756696No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCCCTAGCGTTGTTCGGATTTAC15700.18699202484969177No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT15610.1859200960448209No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC15360.1829425160312908No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC15120.18008403921830188No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCTCGAGCGTTGTTCGGATTTAC15120.18008403921830188No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC14910.1775828720069366No Hit
GTGTCAGCCGCCGCGGTAACACGTAAGTTGCGAGCGTTGTTCGGAATTAT14860.17698735600423054No Hit
GTGTCAGCCGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGAT14840.17674914960314814No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT14810.17639184000152452No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC14680.17484349839448884No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC14280.17007937037284065No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC14050.16733999676039293No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC13810.16448151994740404No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC13800.1643624167468628No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTTCGGATTTAT13620.16221855913712113No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTATCCGGATTTAC13520.16102752713170906No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCGCGAGCGTTGTTCGGATTTAC13360.1591218759230498No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT13330.1587645663214262No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC13280.15816905031872017No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC13070.15566788310735485No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC13020.15507236710464883No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT13020.15507236710464883No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCCCTAGCGTTGTTCGGATTTAC12160.1448294918581052No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAC12010.14304294384998714No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTTACAAGCGTTATCCGGATTTAC11990.1428047374489047No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC11950.1423283246467399No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTTCGGATTTAT11860.14125639584186908No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC11700.1393507446332098No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC11610.13827881582833895No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC11420.13601585501805605No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT11350.1351821326142676No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC11330.1349439262131852No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAT11260.13411020380939678No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGACAAGCGTTATCCGGATTTAC11140.1326809654029023No Hit
GTGCCAGCAGCCGCGGTAAGACAGAGGATGCAAGCGTTATCCGGAATGAT11090.1320854494001963No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCTCGAGCGTTGTTCGGATTTAC10990.13089441739478422No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC10880.12958428218883097No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAC10430.12422463816447675No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC10380.12362912216177073No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC10380.12362912216177073No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC10350.12327181256014712No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTTACAAGCGTTATCCGGATTTAC10320.12291450295852349No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT10010.11922230374174615No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC9990.11898409734066373No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCGCGAGCGTTGTTCGGATTTAC9980.11886499414012254No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAT9970.11874589093958134No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT9950.11850768453849891No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT9500.11314804051414469No Hit
GTGCCAGCAGCCGCGGTAACACGTAAGTTGCGAGCGTTGTTCGGAATTAT9380.11171880210765024No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC9370.11159969890710904No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC9270.11040866690169698No Hit
GAGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT9230.10993225409953217No Hit
GTGCCAGCCGCCGCGGTAACACGTAAGTTGCGAGCGTTGTTCGGAATTAT9170.10921763489628493No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC9130.10874122209412011No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCCCTAGCGTTGTTCGGATTTAC8950.10659736448437843No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC8890.10588274528113119No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT8770.10445350687463674No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT8750.10421530047355432No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC8660.10314337166868348No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT8660.10314337166868348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)