FastQCFastQC Report
Fri 31 Mar 2023
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702594
Sequences flagged as poor quality0
Sequence length300
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC279161.639615786264958No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC246301.4466161633366499No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC216301.270414438204293No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC189031.1102470700589806No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC160960.945380989243472No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC139800.8211000391167831No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT131450.7720572256216103No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC124480.7311196914825261No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT111590.65541168358399No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC107720.632681661041916No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT99560.5847547918059149No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC96460.5665472802089048No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC88830.5217333081169087No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT85230.5005891011010258No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC75790.4451442915927109No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT74610.43821369040417146No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC73540.4319291622077841No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT63050.3703172923198367No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT60610.3559862186757383No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT56320.3307893719818113No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT53000.31128971440049713No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC51390.3018335551517273No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC49460.290497910834879No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT47940.28157035676150627No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC42870.25179226521413794No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC40880.24010421744702493No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC40500.23787232892868176No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT35020.20568614713783792No Hit
GTGTCAGCAGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC32680.19194241257751407No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC31340.18407206885493546No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT30810.1809591717109305No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT28440.1670392354254743No Hit
GTGTCAGCCGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC27720.16281039402229774No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCGAGCGTTGTCCGGATTTAC25790.15147474970544944No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT25080.14730464221065034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23890.14031530711373352No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC23400.13743734560323834No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC22320.1310940834984735No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC20550.12069818171566445No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCGAGCGTTGTCCGGATTTAC20110.11811388974705656No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC19670.11552959777844866No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC18840.11065468338312011No Hit
GTGCCAGCAGCCGCGGTAATACCGGCTGCTCGAGTGATGGCCACTATTAC18290.10742431842236025No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)