FastQCFastQC Report
Fri 31 Mar 2023
Zebra-all.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZebra-all.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558249
Sequences flagged as poor quality0
Sequence length300
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC429337.69065417045082No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC384736.891727526605511No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC290525.204129340133166No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC264664.740895191930482No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC253164.534893927261849No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC231624.149044601960774No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC171893.079091946425341No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC156112.7964223849930763No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT116702.090465007550394No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT106441.9066760531590743No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT97201.7411585152861893No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT87911.5747453197408323No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT68611.229021458166517No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT62901.1267373519701782No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT62351.1168851175729828No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT58371.045590766844186No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT58001.0389629000678908No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC51510.9227065341809837No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT48900.8759532036779286No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC45640.8175563234327334No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT38370.6873276978552582No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC38270.6855363825103135No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC35690.6393204466107418No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT34240.6133463741090446No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT31570.5655182543990227No Hit
GAGTCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC31560.5653391228645283No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC30320.5431268125872146No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT29750.5329163151210302No Hit
GAGTCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC28290.5067631110848385No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC27560.4936865090667426No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC25590.4583975967713332No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC25420.45535236068492735No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC25010.4480079677706543No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC23630.4232878160104183No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC23150.414689502354684No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC22990.4118233978027726No Hit
GAGTCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC20860.3736683809554518No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC20220.3622039627478061No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC20090.35987525279937804No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT18720.3353342325736365No Hit
GAGCCAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC18420.32996028653880255No Hit
GAGTCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC17850.31974978907261814No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT17040.30524013477856654No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC16040.28732698132912016No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC15310.27425037931102425No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGTGTTATCCGGATTTAT15070.26995122248315717No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC14610.26171117189641185No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGTGTTATCCGGATTTAT14340.2568746204650613No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC13920.2493510960162938No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC13620.2439771499814599No Hit
GAGCCAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC13350.23914059855010936No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC12130.21728655134178476No Hit
GAGCCAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC11610.20797171154807262No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT11550.20689692234110588No Hit
GTGTCAGCAGCCGCGGTAAGACAGAGGGTGCAAACGTTGTTCGGAATTAC10940.19596989873694354No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT10670.19113334730559303No Hit
GAGACAGCAGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC10040.1798480606324418No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC9990.17895240295996948No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC9900.1773402191495193No Hit
GTGTCAGCAGCCGCGGTAACACGTAAGTTGCGAGCGTTGTTCGGAATTAT9820.1759071668735636No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC9750.17465324613210234No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT9720.17411585152861894No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTCAT9690.17357845692513557No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC9630.17250366771816877No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGTGTTATCCGGATTTAT9360.16766711628681824No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTCAT9200.16480101173490683No Hit
GAGCCAGCCGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC9190.16462188020041235No Hit
GAGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT8980.16086011797602862No Hit
GTGTCAGCCGCCGCGGTAACACGTAAGTTGCGAGCGTTGTTCGGAATTAT8930.1599644603035563No Hit
GTGTCAGCCGCCGCGGTAAGACAGAGGGTGCAAACGTTGTTCGGAATTAC8910.15960619723456737No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT8760.1569192242171504No Hit
GAGACAGCCGCCGCGGTAATACAGAGGCCAAGAGCGTTGTTCGGATTTAC8450.15136614664782203No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGTGTTATCCGGATTTAT8320.149037436699394No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT8180.1465295952164715No Hit
GAGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT8140.14581306907849365No Hit
GAGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT7980.1429469645265822No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC7850.1406182545781542No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT7380.1321990724569144No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT7310.13094515171545312No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC7220.12933296790500298No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGAATTAT7220.12933296790500298No Hit
GTGTCAGCAGCCGCGGTAACACCGGCAGCTCTAGTGGTAGCTGTTTTTAT7170.12843731023253066No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCGCGAGCGTTGTTCGGATTTAC6990.1252129426116303No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT6800.12180944345623547No Hit
GAGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT6770.12127204885275207No Hit
GTGTCAGCCGCCGCGGTAACACCGGCAGCTCTAGTGGTAGCTGTTTTTAT6650.11912247043881852No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT6640.11894333890432404No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGAATTAT6580.11786854969735729No Hit
GAGACAGCAGCCGCGGTAATACAGAGGTCGCAAGCGTTGTTCGGATTTAC6510.11661462895589603No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT6300.11285286673151228No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTCAT6090.10909110450712854No Hit
GTGCCAGCAGCCGCGGTAAGACAGAGGGTGCAAACGTTGTTCGGAATTAC6070.10873284143813962No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC6050.10837457836915068No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC5940.10640413148971158No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAC5800.1038962900067891No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCGCGAGCGTTGTTCGGATTTAC5710.1022841061963389No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC5610.10049279085139427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)