FastQCFastQC Report
Wed 30 Nov 2022
4061-JW01trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW01trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148632
Sequences flagged as poor quality0
Sequence length231
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACGATGGATACTAGGTGTTGTGAGTATCGACCCTCACAGTG4351929.279697507939073No Hit
TAGCCGTAAACAATGGATACTAGGTGTAGCCTGTATCGACCCGGGCTGTG2079213.988912212713277No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA1841412.38898756660746No Hit
TAGCCGTAAACAATGGATACTAGGTGTAGCCCGTATCGACCCGGGCTGTG104407.0240594219279835No Hit
TAGCCGTAAACGATGGATACTAGGCGTAGCTCGTATCGACCCGAGCTGTG47503.195812476451908No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC19961.3429140427364228No Hit
ACGCCGTAAACGTTGGGCACTAGGTGTGGGTCTCAACCAACGAGATCCGC14330.964126163948544No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG13830.9304860326174714No Hit
TAGCTGTAAACGATGGGCACTAGCTTGGGGTCTCCCTGTGTGATCCCAGG12260.824856020237903No Hit
TAGCCGTAAACGATGGACACTAGGTGTTGGCCGTATCGACCCGGTCAGTG10620.7145163894719845No Hit
TAGCGGTAAACGATGGAAACTAGGTGTTGCCTGTATCGACCCGGGCAGTG9850.6627105872221325No Hit
TAGCTGTAAACGATGAGCACTTGTTTGGAGGGTCCTCCATAGCCTTCCAG5520.3713870498950428No Hit
TAGCTGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA5500.3700414446417999No Hit
ACGCCTTAAACGATGAATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC5070.34111093169707735No Hit
TAGCCGTAAACAATGGATACTAGGTGTTGTGAGTATCGACCCTCACAGTG4750.3195812476451908No Hit
ACGCCGTAAACGATGGGTGCTAGGTGTCGCGGGCTTTGACCCCTGCGGTG3610.24288174821034503No Hit
TAGCCGTAAACGATGGATACTAGGTGTAGCCTGTATCGACCCGGGCTGTG3590.2415361429571021No Hit
ACGCTGTAAACGATGGGTGCTCGGTGTCGCGGGTATCGACCCCTGCGGTG3570.2401905377038592No Hit
ACGCCGTAAACGATGTGTGCTCGGTGTGTGTGGGTAAAACCATGCGCGCC3310.22269766941170138No Hit
TAGCTGTAAACAATGGATACTAGATGTTGGACAGATCGACCTGTGCAGTA3210.21596964314548683No Hit
ACGCCCTAAACTATGGATACTCGACATACGCGATAAACTGTGTGTGTCTG2980.2004951827331934No Hit
ACGCCCTAAACGGTGCACACTAGGTTTTGGAGGATTCGACCCCTTCAGGA2970.19982238010657194No Hit
ACGCCGTAAACGATGTGTGCTCGTTGTCGGGATCTTAGATCCCGGTAACA2660.17896549868130684No Hit
ACGCTGTAAACGATGAGTGCTAGGCGTCGGCACGCTTGCGTGTCGGTGCC2520.1695462619086065No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTGCGTATCGACCCTCACAGTG2450.16483664352225633No Hit
ACGCTGTAAACGATGAACACTAGGTGTCGTGGGTATTGACCCCCGCGGTG2310.15541740674955595No Hit
ATGCCGTAAACGATGTTCACTAGGCGCTGGGTGACTTCCGTCGCTCGGTG2180.14667097260347703No Hit
ACACTGTAAACGATGATTACTCGATGTTGGCGATACACAGCCAGCGTCTT2100.1412885515905054No Hit
TAGCCGTAAACGATGGATACTAGGTGTAGCCCGTATCGACCCGGGCTGTG2030.13657893320415523No Hit
ACGCCGTAAACGATGATAACTAGCTGTTCGGGCTCTACGAGCTTGAGTGG2020.13590613057753378No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGATGGCGTTCGCGTCGTTCCG1990.1338877226976694No Hit
ACGCCCTAAACGATGCGAACTGGACGTTGGGCTCAATTGAGAGTTCAGTG1920.12917810431131924No Hit
ACGCCGTAAACGATGTACACTGGACCGGTGCGATTTTGACGTTGTATCGG1900.1278324990580763No Hit
ACGCCCTAAACGATGTTTACTTGCTGTTTGGCCTTTGGGCTGAGTGGCGA1870.12581409117821196No Hit
TAGCCGTAAACAATGGATACTAGATGTTGACCAGATCGACCTGTGCAGTA1820.12245007804510467No Hit
ACGCTGTAAACGATGAGAGCTAGTTGTCGGCACGCATGCGTGTCGGTGAC1780.11975886753861886No Hit
ACGCTGTAAACGATGAGTGCTCGGTGCTGCGGGTATCGACCCCTGCAGTG1650.11101243339253995No Hit
TAGCCGTAAACAATGGATACTAGATGTTGCACAGATCGACCTGTGCAGTA1550.10428440712632542No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGGGAGTATCGACCCTCACAGTG1510.1015931966198396No Hit
TAGCCGTAAACGGTGCACGTTTGCTGTAAAAGGAATCGACCCCTTTTGTG1490.1002475913665967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCA152.13068E-5224.992619
AACCAAT152.13068E-5224.992618
TCGTGAG600.0224.99261225
CCATAAA152.13068E-5224.992614
TACTACA152.13068E-5224.992615
ACCAATG152.13068E-5224.992619
ACCGTAA152.13068E-5224.992613
CAGTAAA600.0224.992614
AATACCA303.092282E-11224.99261225
CGCCATA152.13068E-5224.992612
TACAACC152.13068E-5224.992618
TCGCCGT800.0224.99261
TGTCAAT100.0019033721224.99265
TTAGGCT100.0019033721224.9926225
AGCCGAT100.0019033721224.99268
CTGTCAA100.0019033721224.99264
ACGCCAT100.0019033721224.99261
CTACTAC100.0019033721224.99264
AGCCCTA750.0224.99262
GGGTCGT100.0019033721224.9926225
Produced by FastQC (version 0.11.9)