FastQCFastQC Report
Wed 30 Nov 2022
4061-JW01trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW01trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148632
Sequences flagged as poor quality0
Sequence length236
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT4405929.643010926314656No Hit
GTTCGCGCTCCCGAAGGCACCCCCTCCTTTCAAAGGGGTTCGCGACATGT3113520.947709779858982No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT1741811.718876150492491No Hit
CTTGGCGCTCCCTAAGGCACTCTCCTCTTTCAAAGAGATTCGCCAGATGT44172.9717692017869637No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC19921.340222832229937No Hit
GCAGGTTACACCCGAAGGCAATCCGCAGACTTTCATCCGCTTAATCCCTG10540.7091339684590129No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT10450.7030787448194198No Hit
CTTCTGGTTCCCTAAGGCACTCTCTCGTTTCCAAGAGATTCCAGAGATGT6880.46288820711556056No Hit
GCAGGATGGTATTGCTACCTCGTTAGGCTTTCACCCTTCTACTACCTGCA6760.45481457559610317No Hit
GTAGAGCCCCGAAGGACACCCTATCTCTAGAGCTTTTCCCTACATGTCAA6310.42453845739813767No Hit
GCACGTTGTACCCGAAGGCCTGGCTCGGCTTTCACCGAGTTAATCCGTGC5510.37071424726842134No Hit
GTACGACCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA5130.3451477474568061No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGCGGATTCGCGACATGT4590.3088164056192475No Hit
GTATACGTTCCAAACGGCACTTTCCTCTTTCAAAGAAATTCGTATCATGT4530.3047795898595188No Hit
ATCCTAGCCAGCCGAACTGAAGGAAATCATCTCTGAAATCCATACTAGGT3170.213278432639001No Hit
CTGGCAGTGTATTGCTACAAAGTGAGCTTTCACCCACGGTCTCCCAGCAT3060.205877603746165No Hit
GCACCGGTCCCTTGCGGGAAAGACGCCTTTCAGCGTCGGTCCAGTGCATG2600.17492868292157812No Hit
TACGCAGTCCCGAAGGACGATCCCCTTTCAGTTCACTCCCACGCACATTC2570.17291027504171377No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAAGGGGTTCGCGACATGT2320.1560902093761774No Hit
GTTCGTGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCACGACATGT2280.1533989988696916No Hit
GCAGATGTCCTTGCGGAAGCCGACTTTCGCCGGATGTCATCTACAGTTCA2220.14936218310996285No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGACATTCGTATCATGT2190.1473437752300985No Hit
CACTAATCCAGCCGAACTGAAGGAATCCATCTCTGGAATCCGCGATTAGG2150.14465256472361268No Hit
GTATACGTTCCAAAAGGCACTTTTTTCTTTCGAAAAAATTCGTATCATGT2100.1412885515905054No Hit
GTTCGCGCTCCCGAAGGCACCCCCTCCTTTCAAAGGGGCTCGCGACATGT2100.1412885515905054No Hit
GACGGCTCCCGAAGGTCAGTCACGTTTCCGCAACCTACCACCGCCATGTC2060.13859734108401958No Hit
GCATGTTCCGGTCTTGCGACTGTGGCTCTACTTTCATAGAGTTGATCCAT2000.13456052532429086No Hit
GTACGGCCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA1990.1338877226976694No Hit
ATGGCGGCCCCCGAAGGGGAATACCTGTTTCCAGATACGTCCACCACAGT1930.12985090693794069No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGCAATTCGTATCATGT1920.12917810431131924No Hit
CTCACGGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCCGTGGATGT1790.12043167016524033No Hit
CTACAGGTTCTCCGAAGAGCACCCCGATCTTTCGACCAGGCTCCTGCATT1750.11774045965875451No Hit
CCAGCCGCCCGAAGGCTCTCAAAGTTTCCTCTGAGATTCGTCTGGATTTC1650.11101243339253995No Hit
GCAAGGCCCCGAAGGACACCGTATCTCTACGGCTTTTCCCTGCATGTCAA1640.1103396307659185No Hit
AGCACACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGTGCTATGTCA1570.10563001237956834No Hit
CTACAGGTTCTCCGAAGAGCACCCCAATTTTTCAACCAGGTTCCTGCATT1560.10495720975294688No Hit
CCACTAGCTCCCTTGCGGGCACATCAGACTTTCATCCGACTTCTAATGAC1550.10428440712632542No Hit
GTTCGCGCTCCCGAAGGCACCCCCTCCTTTCAAGAGGATTCGCGACATGT1520.10226599924646104No Hit
GTATCCGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT1500.10092039399321816No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCTACATGT1490.1002475913665967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG400.0229.9953230
CAGCGGC202.1340202E-7229.99532
GCAAGTT202.1340202E-7229.99531
TGCCATT100.0017821094229.99536
CCGATTC100.0017821094229.99534
ATGGCAT100.0017821094229.99536
TATGGGT100.0017821094229.99532
TGGCGGC450.0229.99532
TTGTCCT202.1340202E-7229.99535
ATGTCCT202.1340202E-7229.99535
ATGTCCC100.0017821094229.99535
TAGCCGT100.0017821094229.99532
GTAGAGC800.0229.99531
CGGATGG100.0017821094229.99533
GGTATTG1350.0229.99538
GACGCTC100.0017821094229.99532
TTCATGT100.0017821094229.99532
ATCGGGT100.0017821094229.99532
GATTCAG100.0017821094229.99535
CCGCCAG202.1340202E-7229.99535
Produced by FastQC (version 0.11.9)