FastQCFastQC Report
Wed 30 Nov 2022
4061-JW02trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW02trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169030
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA8968353.05744542388925No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA161979.582322664615749No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG45102.668165414423475No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTGAGTATCGACCCTCACAGTG26801.5855173637815771No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA23361.3820031946991658No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG15500.9169969827841212No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA11400.6744364905638053No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA8750.5176595870555523No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG7740.45790688043542566No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG6190.36620718215701353No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG5080.3005383659705378No Hit
GGAAAGGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT4850.2869313139679347No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT4530.26799976335561737No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTCATACTGGTCGGTGC4210.24906821274330002No Hit
ACGCTGTAAACGTTGGGTACTTGGTGTTGGGGGTTTGAGTCCCTCAGTGC4200.2484766017866651No Hit
ACGCCCTAAACAATGTTCACTTGGTGTCGGCCGCTTATACTGGTCGGTGC3920.23191149500088742No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA3370.199372892385967No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3260.1928651718629829No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3240.19168194994971308No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2990.17689167603384015No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2700.15973495829142756No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA2680.15855173637815773No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCTGGGATGGTGCGACCGTTTCAG2580.15263562681180853No Hit
ACGCCGTAAACGATGAATACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2500.14790273915872923No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2380.14080340767911023No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC2300.13607052002603087No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2300.13607052002603087No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG2260.1337040761994912No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2220.13133763237295154No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2170.12837957758977694No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA2170.12837957758977694No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2150.1271963556765071No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA2000.11832219132698338No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG1970.11654735845707863No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG1960.11595574750044371No Hit
TGGCTGTAAACGATGAGCACTAGGTGTCAGGGGCTTCGACCCCCTTGGCG1950.1153641365438088No Hit
CAGCTGTAAACTATGCTCGCTAGGTGTCAGGGACGGTGCGACCGTTTCTG1940.11477252558717388No Hit
TAGCCCTAAACGATGAGCGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG1940.11477252558717388No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1930.11418091463053896No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1930.11418091463053896No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1900.11240608176063421No Hit
TAGCCGTAAACGATGTGAACTTGGCGTCGGTGGGTTAAACTCCATCGGTG1830.10826480506418978No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1800.10648997219428503No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG1750.10353191741111045No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA1750.10353191741111045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAATC152.1309168E-5225.0225
AACAATG2350.0225.09
AACGTAT202.3818575E-7225.0225
CTGTACA100.0019034148225.04
CGACGTA202.3818575E-7225.02
AGCCCTA1200.0225.02
CCCTAAA5800.0225.04
ACCGCTT100.0019034148225.09
CACGATG252.6775524E-9225.09
CACTGTA202.3818575E-7225.02
TAGCCAT100.0019034148225.01
TGGCCCT600.0225.01
AAACGCT1950.0225.08
TAACCGC100.0019034148225.07
CCATAAA202.3818575E-7225.04
AACGATC152.1309168E-5225.09
AGCTGTC100.0019034148225.0225
CGTACAC202.3818575E-7225.05
TTAACAA252.6775524E-9225.0225
CGTAACC202.3818575E-7225.05
Produced by FastQC (version 0.11.9)