FastQCFastQC Report
Wed 30 Nov 2022
4061-JW02trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW02trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169030
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA7508944.42347512275928No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA163539.674613973850796No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA60283.566230846595279No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA56173.3230787434183284No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC38232.261728687215287No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA26241.552387150210022No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT23901.4139501863574513No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC13790.8158315091995504No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7250.4289179435603147No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT6390.3780394012897119No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5620.3324853576288233No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC5300.3135538070165059No Hit
ACTAGCTCTCTACCCGAAGGCAGAGTCGTCCCCCTTTCAGGTTCCTACCA5220.3088209193634266No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCC4670.27628231674850623No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3590.21238833343193517No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC3100.18339939655682422No Hit
TCAGCGGTCCCTTGCGGGAGGGCTCTATCTCTAGAGATTTCCACTGCAGT2950.17452523220730048No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG2830.1674259007276815No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA2680.15855173637815773No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG2280.13488729811276104No Hit
CAGCGATTCAGGTAAGACCTTCAGCCTGACCTACATATTGCTGTCGCCCC2270.13429568715612614No Hit
ACTAGCTCTCTACCCGAAGGCAGAGTCGGCCCCCTTTCAGGTTTCTACCA2270.13429568715612614No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2110.12482991184996745No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA1920.11358930367390405No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA1910.11299769271726913No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1860.11003963793409453No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1850.10944802697745962No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1760.10412352836774537No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC1750.10353191741111045No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC1750.10353191741111045No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATATAACCGTCGCCCC1720.10175708454120569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGG100.001782216230.000026
AAACTGT100.001782216230.000022
GAGGACA100.001782216230.00002230
GCAAGCG100.001782216230.000021
TTGGACG100.001782216230.00002230
CAAGTAG950.0230.000029
AATGGCT100.001782216230.000021
GCGCATT100.001782216230.000023
TGACGAC100.001782216230.000022
ATACTGG202.134675E-7230.000023
AGCATGG100.001782216230.00002230
TACTGGT202.134675E-7230.000024
TGACAGC100.001782216230.000022
GCAGGGC800.0230.000021
GTTCAGG100.001782216230.000021
GTTCAGA100.001782216230.000021
GCTACTG100.001782216230.000025
GACGACC100.001782216230.000023
TATACGT800.0230.000022
ATGCCCT100.001782216230.000025
Produced by FastQC (version 0.11.9)