FastQCFastQC Report
Wed 30 Nov 2022
4061-JW03trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW03trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150917
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA7252048.052903251456094No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA94786.28027326278683No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA56133.7192629061007043No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA38682.562998204310979No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG18441.2218636734098876No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG14070.9323005360562427No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG8790.5824393540820451No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC8580.5685244207080714No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG8400.5565973349589509No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG7250.48039650933957073No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT7080.4691320394654015No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG7050.4671441918405481No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG6470.42871247109338245No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT6210.411484458344653No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA5930.39293121384602125No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG4500.2981771437280094No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA4270.2829369786041334No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG4210.2789612833544266No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGTGGGAGTTGACCCCTGCCGTG3810.2524566483563813No Hit
TAGCCGTAAACTATGGATACTGGATCTTGCATATATTTGCTTGGTCGAAG3640.24119217848221208No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3570.23655386735755415No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA3530.2339034038577496No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG3370.2233015498585315No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG3270.2166753911090202No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG3240.21468754348416677No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3070.20342307360999753No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG3040.20143522598514416No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2820.18685767673621925No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2780.1842072132364147No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2630.17426797511214773No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2570.17029227986244094No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2430.16101565761312508No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2310.15306426711371152No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA2250.1490885718640047No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG2190.14511287661429792No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2150.1424624131144934No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA2130.14113718136459114No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2050.1358362543649821No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG2040.13517363849003095No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2030.1345110226150798No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTGGGGCTCGCAAGGGTTCCGTGCC1950.12921009561547075No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1900.1258970162407151No Hit
TAGCCGTAAACGATGGATACTAGGTGTTAGAGGTATCGACCCCTCTAGTG1870.1239091686158617No Hit
ACGCTGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1810.11993347336615491No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1740.11529516224149698No Hit
TGGCCGTAAACGATGAGCACTAGGTGTGGGAGGTATCGACTCCTCCTGTG1730.11463254636654585No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG1720.11396993049159472No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1680.11131946699179018No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGAGGTATTGATCCCTTCGGTG1640.10866900349198567No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT1590.10535592411723No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1590.10535592411723No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG1540.10204284474247434No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTTTCGCA1510.10005499711762095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA750.0225.000022
TAGCCCT750.0225.000021
TCGCCGT100.0019032137225.01
TGCCCTA100.0019032137225.02
ACGCCAT202.3810207E-7225.01
CGACGTA252.6757334E-9225.02
GACGTAA252.6757334E-9225.03
ACCGATG100.0019032137225.09
ACCCTAA152.130466E-5225.03
GCCATAA252.6757334E-9225.03
GTAATCG100.0019032137225.06
AAACGCT1150.0225.08
AGGCCGT100.0019032137225.01
CGAATTC252.6757334E-9225.0225
AACGATA1700.0225.09
AACTGTG252.6757334E-9225.09
TTAACAA100.0019032137225.0225
AGTTAGC350.0225.0225
CATAAAC202.3810207E-7225.05
CGTAAAT303.092282E-11225.05
Produced by FastQC (version 0.11.9)