FastQCFastQC Report
Wed 30 Nov 2022
4061-JW03trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW03trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150917
Sequences flagged as poor quality0
Sequence length236
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA5403335.80312357123452No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1573410.425598176481111No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1512610.022727724510824No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA32972.1846445397138825No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC13120.8693520279358854No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC10390.6884578940742262No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT9910.6566523320765719No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7680.5088889919624694No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5130.33992194384993074No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA4950.32799485810081036No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4380.29022575322859584No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC4070.26968466110511075No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA4060.2690220452301596No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA3990.2643837341055017No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT3930.26040803885579494No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA3780.25046880073152794No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA3510.23257817210784737No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT2810.1861950608612681No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2580.1709548957373921No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2570.17029227986244094No Hit
GCAGGTTGCTCCAAAGGAGCATCTTTAGCCCTTTCAGGTAAATATGCCTA2570.17029227986244094No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC2570.17029227986244094No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT2480.16432873698788075No Hit
CTTACGGCTCCCCGAAAGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT2400.1590278099882717No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2370.1570399623634183No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG2320.15372688298866266No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC2290.15173903536380925No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2290.15173903536380925No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG2240.1484259559890536No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT2200.14577549248924906No Hit
ACAGGTTTCTCCAAAGGAGAATCACTATCCCTTTCAGGTAGCTATGCCTG2180.14445026073934678No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2120.14047456548964No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA2100.13914933373973773No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG2090.13848671786478658No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT2060.1364988702399332No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2000.13252317499022642No Hit
GTAACAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCATTACAGTTC1920.12722224799061735No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA1830.12125870511605716No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA1820.12059608924110604No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1790.11860824161625265No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1720.11396993049159472No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1700.11264469874169246No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1670.11065685111683907No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1660.10999423524188792No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1640.10866900349198567No Hit
TACAGCGTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1610.10668115586713227No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA1610.10668115586713227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG100.0017820274230.000023
GAGGACG100.0017820274230.00002230
AGTCCGC100.0017820274230.000022
TTGGACT202.133911E-7230.00002230
TGCCATT202.133911E-7230.000026
CAGCCGC100.0017820274230.000022
CATTGTC100.0017820274230.000024
CAGCAAG100.0017820274230.000022
TTGTCGA100.0017820274230.000025
GGTCCGA100.0017820274230.000026
ATGCTGC100.0017820274230.000023
ATTCGGT400.0230.000027
TAAGTAA202.133911E-7230.000029
AGGCGAT100.0017820274230.000027
ACTAGCT100.0017820274230.000021
GATGGTG100.0017820274230.000021
TGTTAGG100.0017820274230.000026
AACTGTT202.133911E-7230.000023
AGCGATT2350.0230.000022
ATAAGCT100.0017820274230.000021
Produced by FastQC (version 0.11.9)