FastQCFastQC Report
Wed 30 Nov 2022
4061-JW04trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW04trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93207
Sequences flagged as poor quality0
Sequence length231
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA2695528.919501754160095No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA45234.852639823189246No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA41954.500734923342668No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG26752.8699561191755985No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA17931.9236752604418124No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG14531.558895791088652No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG12241.3132060896713766No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG9531.0224553949810637No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG8420.9033656270451791No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG7320.7853487399015097No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT5980.6415827137446759No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC5220.5600437735363224No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG4790.5139098994710698No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG4310.4624116214447413No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG4270.4581200982758805No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG4020.4312980784705011No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT3750.40233029708069135No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG3470.3722896348986664No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3220.345467615093287No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2820.30255238340467994No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2690.2886049331058826No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2670.2864591715214523No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2670.2864591715214523No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2630.28216764835259156No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2580.27680324439151566No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG2430.260710032508288No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2390.2564185093394273No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2380.2553456285472121No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2370.2542727477549969No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG2290.24568970141727553No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG2030.21779480081968094No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG2010.21564903923525056No Hit
ACGCTGTAAACGATGAATACTAGGTGTTGGGTTTTAAACTCAGTGCCGCA1970.2113575160663899No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG1970.2113575160663899No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG1930.20706599289752914No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1850.19848294655980775No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA1830.19633718497537742No Hit
ACGCCGTAAACGATGATTACTCGTTGTTGGCGATATATTGTCAGCGACTG1790.1920456618065167No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGGGGTATTGATCCCTTCGGTG1770.18989990022208633No Hit
ACGCCGTAAACGATGAGTACTAGGTGTCGGGGGTTACCCCCCTCGGTGCC1760.18882701942987115No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1740.1866812578454408No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG1720.18453549626101046No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG1720.18453549626101046No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1680.18024397309214973No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG1660.17809821150771937No Hit
ACGCCGGAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1650.1770253307155042No Hit
ACGCCGTAAACGATGAATGCCAGCCGTTAGTGGGTTTACTCACTAGTGGC1620.17380668833885865No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG1610.1727338075466435No Hit
ACGCTGTAAACGATGAGTGCTTGGTGTAGCGGGTATCGACCCCTGCTGTG1560.16736940358556762No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1560.16736940358556762No Hit
ACGCTGTAAACGTTGGGTACTTGGTGTTGGGGGTTTGAGTCCCTCAGTGC1510.16200499962449172No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG1510.16200499962449172No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGAGGTATTGATCCCTTCGGTG1470.15771347645563102No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC1370.14698466853347925No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1330.14269314536461852No Hit
ACGCCGTAAACGATGAATACTAGGTGTCGGGGGTCGAACCTCGGTGCCGC1320.14162026457240337No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG1310.1405473837801882No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1280.13732874140354265No Hit
ACGCCGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCCCTCAGTG1250.13411009902689713No Hit
ACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1240.13303721823468195No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1240.13303721823468195No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG1210.1298185758580364No Hit
ACGCTGTAAACTTTGGGCACTAGGTATTGGAGGTCTCAACCCCTTCAGTG1150.12338129110474536No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1130.121235529520315No Hit
TAGCCGTAAACGATGTAGACTTGGTGTTGGAGGGGTAAAAGCCTTCAGTG1130.121235529520315No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGATCAGTGTCGCA1120.12016264872809983No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGTGGGAGTTGACCCCTGCCGTG1110.11908976793588465No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC1110.11908976793588465No Hit
ACGCCCTAAACGATGGGCACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1110.11908976793588465No Hit
TAGCCGTAAACGATGGATACTAGGTGTTAGAGGTATCGACCCCTCTAGTG1090.11694400635145429No Hit
ACGCCGTAAACGATGAATACTAGGTGTTGGGGGTCGAACCTCGGTGCCGC1080.11587112555923912No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCATCGAG1040.1115796023903784No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGCCATATTGTTCAGTGTCGCA1020.10943384080594806No Hit
GCACAGTAAACGATGTGCACTAGGTGTTGGGCCGAGCGGTTCAGTGCCGA980.10514231763708735No Hit
CAGCTGTAAACGATGCTAGCTAGGTGTTGGGTCGGCCACGCGCCGCCCCA970.10406943684487216No Hit
ACGCCGTAAACGGTGAGCACTAGGTGTAGCGGGTATTGACCCCTGCTGTG970.10406943684487216No Hit
TAGCCGTAAACGATGGACACTAGATGTTCTCGCGTTCATGCGGGAGTGTC970.10406943684487216No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG940.10085079446822663No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGGATTTGCTTGGTCGTAG940.10085079446822663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG350.0225.09
AAGTAAA100.0019020495225.04
CTGTCAA100.0019020495225.04
ACCATAA100.0019020495225.03
TAAACAA303.092282E-11225.07
CTGACTC100.0019020495225.0225
GGCCCTA500.0225.02
GCCATAA202.3761822E-7225.03
AAACGCT800.0225.08
AGACGAT303.092282E-11225.08
AACGATC202.3761822E-7225.09
AACGATA2800.0225.09
ACTGTCA100.0019020495225.03
CGTAACG100.0019020495225.05
CGTAACC100.0019020495225.05
CGTAAAA450.0225.05
CCGAATC202.3761822E-7225.0225
ACACTGT1050.0225.01
ACCGTAA202.3761822E-7225.03
ACGTAAA202.3761822E-7225.04
Produced by FastQC (version 0.11.9)