FastQCFastQC Report
Wed 30 Nov 2022
4061-JW04trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW04trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93207
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1697318.2100056862682No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA963710.33935219457766No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA87649.40272726297381No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA59526.385786475264734No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC20762.227300524638707No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC20292.1768751274045943No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC9601.0299655605265698No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT9591.0288926797343547No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC8100.8690334416942934No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC6880.7381419850440418No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT6750.7241945347452445No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC6120.6566030448356883No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA5500.5900844357183473No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT4730.5074726147177787No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT4070.43666248243157707No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3840.41198622421062797No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG3370.36156082697651465No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC2990.32079135687233795No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2980.3197184760801227No Hit
CACCCTGTCCCGAAGGACTTCGCCCATCTCTGGGTTATGCAGGGGATGTC2910.3122083105346165No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT2590.27787612518373084No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA2470.26500155567714867No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA2430.260710032508288No Hit
GCTCCAGGCTCCGAAGAGAAGGTCACATCTCTGCGACCGGTCCTGGACAT2370.2542727477549969No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2260.24247105904063No Hit
ACGAAGGCGTATTGCTACGCTGCGATATCGCTACCGCATTCCTCCGTATG2240.24032529745619965No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA2210.23710665507955409No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC2190.23496089349512375No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2170.23281513191069342No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2150.23066937032626303No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA2130.2285236087418327No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT2130.2285236087418327No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA2070.2220863239885416No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2000.21457615844303538No Hit
CTCCAGGTTCTCTCTAATGAAAGCACTCTCCTGTTTCCAGGAGATTCCCG1950.2092117544819595No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1930.20706599289752914No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA1840.19741006576759257No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC1770.18989990022208633No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA1750.18775413863765597No Hit
GCAGCAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCACTGCAGTTC1710.18346261546879525No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCACTTCCCTACCACTTGCATG1680.18024397309214973No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGTTATGCCTA1650.1770253307155042No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1640.175952449923289No Hit
ACAGCAGCCCCGAAGGGAAGGGTACTTTCATACCCGGTCTACTGCATTTC1600.17166092675442832No Hit
ACACCTGCTCCCGAAGGAGGGACTCCTATTTCTAAGAGTTTACAGGTGCG1570.16844228437778277No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1570.16844228437778277No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT1560.16736940358556762No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1540.16522364200113726No Hit
GCCGGCTCCCAGGAAACCTGGGTCCTCGCCCTTTCGGGTCCGTACCACCG1520.1630778804167069No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC1480.15878635724784618No Hit
CTTACGGCTCCCCGAAAGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT1480.15878635724784618No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1470.15771347645563102No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1450.15556771487120066No Hit
TCAGCGGTCCCTTGCGGGAGGGCTCTATCTCTAGAGATTTCCACTGCAGT1420.15234907249455512No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC1420.15234907249455512No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1400.1502033109101248No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1390.14913043011790958No Hit
TAACCTGCCAATTGCTTGGAAATACCCTTTCAGGTACGGTCACGCTACAT1340.14376602615683373No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT1340.14376602615683373No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1320.14162026457240337No Hit
ACAGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCTGTATGTC1290.13840162219575783No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA1290.13840162219575783No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACTTGCATG1280.13732874140354265No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCGCCCC1270.1362558606113275No Hit
CAGCTAGTCAAGCAGAGTCTTCAGCCCGGCTATCATTCAGCTGTCTTATC1240.13303721823468195No Hit
ACGGAGGCGTATTGCTACGCTGAGACATCGCTGCCCCATTCCTCCGTATG1210.1298185758580364No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC1150.12338129110474536No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1140.12230841031253019No Hit
TACAGTTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAACTGCAATATCA1130.121235529520315No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC1090.11694400635145429No Hit
ATACATGTCCGAAGAAGGGTGTATCTCTACACCTGTCATGTACAGTTCAA1080.11587112555923912No Hit
GGCCGCGCCTCTTGCGAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1040.1115796023903784No Hit
TCAGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCTGTATG1030.11050672159816323No Hit
GCAGGGCCCTCTCGGACGCGACGTTTCCGCCGTTTTACCCTGCATGTCAA1030.11050672159816323No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC1030.11050672159816323No Hit
CTCCGGGTTCTCTCTAATGAAAGCACTCTCCTGTTTCCAGGAAATTCCCG990.10621519842930252No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA980.10514231763708735No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCATACTGCTGTCGCTCC950.10192367526044183No Hit
GTGACAGCTCCTTGCGGAGAGAGTGGCTTTCACCACCTGTCTGCCCCTCT950.10192367526044183No Hit
ACACGGGTCCCGAAGGAAGACTGTATCTCTACAGCTGTCCCGTGCGGTTC950.10192367526044183No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA940.10085079446822663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAA2650.0229.996261
CGCCGAA6950.0229.996268
TCCGGAT100.0017810247229.996258
CTTGTCT400.0229.996254
CAGGTCT151.9493544E-5229.996252
CAGCGAT3700.0229.996251
TAGAGAC100.0017810247229.996252
AAATCGG100.0017810247229.996255
AAATCAG400.0229.996255
CCGGGAA100.0017810247229.996259
GCAAGGG100.0017810247229.996251
ACGCCTC151.9493544E-5229.996252
AATCAGG400.0229.996256
GAGCTGT100.0017810247229.996255
TTGGAGC100.0017810247229.996252
GTCTGGT400.0229.996257
CTATCTG151.9493544E-5229.996251
CGAAAAA151.9493544E-5229.996251
ACGTTGT100.0017810247229.996253
ACGTTCC151.9493544E-5229.996253
Produced by FastQC (version 0.11.9)