FastQCFastQC Report
Wed 30 Nov 2022
4061-JW05trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW05trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146784
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA8099055.176313494658814No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA104607.126117287987792No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG49533.374345977763244No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA24341.658218879441901No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA18031.2283355134074558No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG17271.1765587529976018No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT8680.591345105733595No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT6660.45372792674950946No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG6000.40876389797253104No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG5840.39786352735993025No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG4740.3229234793982995No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG4710.32087965990843687No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG4360.29703509919337256No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT3850.26229016786570747No Hit
ACGCCGTAAACGATGAGTGCTAAGTGTTGGAGGGTTTCCACCCTTCAGTG3710.25275234357968174No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG3570.24321451929365598No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG3320.22618269021146717No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG3160.21528231959886635No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3160.21528231959886635No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA3150.2146010464355788No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG3130.2132385001090037No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA2790.19007521255722695No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG2760.1880313930673643No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2510.1709995639851755No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2430.16554937867887506No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG2260.1539677349029867No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2220.1512426422498365No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2220.1512426422498365No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG2190.14919882275997384No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2160.1471550032701112No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2140.14579245694353607No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2110.14374863745367342No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG2050.1396609984739481No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG2040.13897972531066058No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2030.13829845214737302No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG2000.13625463265751037No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1950.1328482668410726No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG1890.1287606278613473No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG1820.12399171571833441No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1810.12331044255504686No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1790.12194789622847176No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC1770.12058534990189665No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1770.12058534990189665No Hit
CAGCTGTAAACTATGCTCGCTAGGTGTCAGGGACGGTGCGACCGTTTCTG1730.11786025724874645No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1720.1171789840854589No Hit
ACGCCGTAAACGATGAGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1680.11445389143230869No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG1570.10695988663614563No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTTTCGCA1550.10559734030957052No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1530.10423479398299543No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC1520.10355352081970787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAA100.0019032551224.996284
GACGTAA202.3810026E-7224.996283
TTGTCTA100.0019032551224.99628225
TAAACCA100.0019032551224.996287
GCACCAT100.0019032551224.996281
AGACGAT100.0019032551224.996288
CTTGCAC100.0019032551224.99628225
GTCGTAA100.0019032551224.996283
TCGTAAA100.0019032551224.996284
CGTAACC100.0019032551224.996285
CACCGTA202.3810026E-7224.996282
CAATACA100.0019032551224.99628225
AACAGAT100.0019032551224.996288
GTAAATG100.0019032551224.996286
CTCAGTC100.0019032551224.99628225
AGTCGTA100.0019032551224.99628225
CACCATA100.0019032551224.996282
AACACTG202.3810026E-7224.996289
CCCTAAA2150.0224.996264
TAAACAA450.0224.996267
Produced by FastQC (version 0.11.9)