FastQCFastQC Report
Wed 30 Nov 2022
4061-JW05trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW05trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146784
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA7433650.643121866143446No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA131298.944435360802268No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA62694.270901460649662No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC39652.7012480924351427No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA17431.1874591236102028No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC8390.571588183998256No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA6250.4257957270547199No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC6110.4162579027686942No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA5180.35289949858295183No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC4980.3392740353172008No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC4900.33382385001090037No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC4290.2922661870503597No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT3490.23776433398735555No Hit
CACTTTGTCCCCGAAGGGAAAGTCCTATCTCTAGGATTGTCAAAGGATGT3260.22209505123174186No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC3240.22073250490516677No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3020.20574449531284064No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG2780.1893939393939394No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA2670.18189993459777634No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2580.17576847612818836No Hit
TACAGCTTCCCGAAGGACCTCCCCTCTCAGGGAGTTTAGCTGCAATATCA2140.14579245694353607No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2120.14442991061696098No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT2060.14034227163723567No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2000.13625463265751037No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1920.13080444735120994No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT1800.12262916939175932No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC1660.11309134510573358No Hit
TACAGCGTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1640.11172879877915849No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT1640.11172879877915849No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1600.10900370612600828No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC1590.10832243296272073No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1560.10627861347285808No Hit
GCAGGTTGCTCCAAAGGAGCATCTTTAGCCCTTTCAGGTAAATATGCCTA1490.10150970132984521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCTG100.0017804127230.06755
AGATAAC100.0017804127230.06756
GGTCCGA100.0017804127230.06756
GCGCCTA202.1306005E-7230.06755
TTCGTAA100.0017804127230.06759
AATGGCT100.0017804127230.06751
CGATTCT202.1306005E-7230.06754
GTAGGCT100.0017804127230.06751
TGAAAAT100.0017804127230.06752
CCAGATA100.0017804127230.06754
CTGAAAA100.0017804127230.06751
GCTCTCC202.1306005E-7230.06755
TCTACCC100.0017804127230.06759
CAGATAA100.0017804127230.06755
AAACCGA100.0017804127230.06751
CCATAAC100.0017804127230.06756
AGCGATG100.0017804127230.06752
TCCCGTA202.1306005E-7230.06758
ATAACTA100.0017804127230.06758
CCAACCA100.0017804127230.06754
Produced by FastQC (version 0.11.9)