FastQCFastQC Report
Wed 30 Nov 2022
4061-JW06trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW06trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192443
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA11486859.68936256449962No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA152037.900001558903155No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG34081.77091398492021No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT31801.6524373450839989No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA20231.0512203613537516No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA13330.6926726355336386No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG8550.44428739938579215No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT7780.40427555172180857No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT7490.38920615454965884No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG5920.307623556065952No Hit
ACGCCGTAAACGATGAGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG5870.3050253841397193No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG5520.28683818065609035No Hit
CAGCTGTAAACTATGCTCGCTAGGTGTCAGGGACGGTGCGACCGTTTCTG5050.262415364549503No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG4980.2587779238527772No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG4960.2577386550822841No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA4840.2515030424593256No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA4680.243188892295381No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA4430.23019803266421746No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA4220.21928571057404012No Hit
ACGCGGTAAACGATGAATACTAGGTGTAGGGGGTATCAACTCCCCCTGTG3670.1907058193854804No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC3590.18654874430350804No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC3530.1834309379920288No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3520.18291130360678226No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA3470.18031313168054955No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA3470.18031313168054955No Hit
TAGCTGTAAACGATGTAGACTTGGCGTAGGTTGGGTAAAACCGATCTGTG3470.18031313168054955No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG2860.1486154341805106No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT2850.14809579979526405No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG2750.14289945594279865No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG2740.1423798215575521No Hit
GGAAAGGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT2590.134585305778854No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2590.134585305778854No Hit
ACGCCGTAAACTATCCCTGTTAGGTGTTTGGTGGTTCACTGAGATTTTTT2590.134585305778854No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2590.134585305778854No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGGATTTGCTTGGTCGTAG2490.1293889619263886No Hit
TGGCAGTAAACGATGTGAACTTGGCGTTGGTGGGATAAAACCTATCAGTG2480.12886932754114205No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG2450.12731042438540244No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2430.12627115561490934No Hit
ACGCTGTAAACAATCCCTGCTAGATGTTGGTTTGACTCTTGTTTTTACTT2420.1257515212296628No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT2390.12419261807392319No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG2310.12003554299195086No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2290.1189962742214578No Hit
TAGCCGTAAACGATGGGTACTAGGTGTGGGGGGTGTCAACTCCTCCCGTG2260.11743737106571815No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2140.11120175844275967No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG2120.1101624896722666No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG2030.10548578020504772No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2010.10444651143455466No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA2010.10444651143455466No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCTGGGATGGTGCGACCGTTTCAG2000.10392687704930811No Hit
TAGCTGTAAACAATCCCTATTAGATATTTGTCTTAAATTGCCGTATTTGA1950.10132870512307542No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1950.10132870512307542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT100.0019036654224.998181
GGCAGAG252.6775524E-9224.99818225
AGCCCTA800.0224.998182
AGCTTTA100.0019036654224.99818225
TAGCGGT100.0019036654224.998181
ACCGATG350.0224.998189
TGGCAGT650.0224.998181
AAACGCT1750.0224.998188
AAACGAC100.0019036654224.998188
CGTAGAC202.3828215E-7224.998185
CCATAAA100.0019036654224.998184
AAACACT3650.0224.998188
ACCGTAA202.3828215E-7224.998183
TCATAGC202.3828215E-7224.99818225
CTGATTT750.0224.99818225
AACACTG252.6775524E-9224.998189
CACGGTA303.092282E-11224.998172
CGTGCGT152.1314429E-5224.99817225
GGACTGC152.1314429E-5224.99817225
TGGCCCT303.092282E-11224.998171
Produced by FastQC (version 0.11.9)