FastQCFastQC Report
Wed 30 Nov 2022
4061-JW06trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW06trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192443
Sequences flagged as poor quality0
Sequence length236
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA10613455.15087584375633No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA149787.783083822222685No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA49942.5950541199212234No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT30451.5822867030757157No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC29901.5537068118871562No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA26021.3520886704114985No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA23871.2403672775834924No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA8160.42402165836117706No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC6800.3533513819676476No Hit
CTCCCTGCCCCGAAGGGCTTCCTCCGTTACGAGTAATTCAGGGGATGTCA6030.3133395343036639No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC5920.307623556065952No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA5470.2842400087298577No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4950.25721902069703756No Hit
CAGCGATTCTGGTAAAGTCTTTAGCTTGACCTACATATTGCTGTCGCCCC4690.24370852668062754No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC4240.2203249793445332No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA3840.19953960393467154No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT3710.19278435692646653No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC3630.1886272818444942No Hit
CTCACTTCCTCCGAAGAGACCGCATGTTTCCATGCGTTTAAGTGGATGTC3420.17771495975431686No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT3300.17147934713135837No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC2950.15329214364772947No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2950.15329214364772947No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA2940.15277250926248293No Hit
ACAAGCTCCCCGAAGGGTCGGTCACCTTTCGGTTTCCTACCACTTGTATG2920.15173324049198983No Hit
ATAGCACCCTCGAAGGCTGAATCCATTTCTGGAAACGTGCAGCTATAGTT2880.14965470295100367No Hit
GCATGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACATGCATG2800.14549762786903134No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC2620.13614420893459364No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCC2610.1356245745493471No Hit
GTAGCACCCTCGAAGGCTGTTCCTCTTTCAAGAAACATGCAGCTACAGTT2330.12107481176244395No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT2220.115358833524732No Hit
ATAGCGATCTCGAAGACGTTTCATATTTCTACAAAACCACAGCTATAGTT2180.11328029598374585No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG2150.11172139282800621No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC2140.11120175844275967No Hit
GCAAAGCCCTTTCGGACACGACGTTTCCGCCGCTTTTCCTTGCATGTCAA2100.10912322090177351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG350.0229.99818230
CTGCTAG151.9525069E-5229.99818230
AAACTGT252.3483153E-9229.998182
ATGGGCC151.9525069E-5229.998182
TTAGGAA151.9525069E-5229.998188
AAATCTC151.9525069E-5229.99818230
AGATAAC252.3483153E-9229.998186
CGAAAAA151.9525069E-5229.998181
TCCTGAA252.3483153E-9229.998188
ACTCTCG302.5465852E-11229.998186
CGCGCTC151.9525069E-5229.998184
GGTCCAG252.3483153E-9229.998185
ATAGCAC350.0229.998181
ATTGTCC151.9525069E-5229.998185
CACGAAG151.9525069E-5229.998189
GTGGCGT151.9525069E-5229.99818230
GTGGCAC151.9525069E-5229.99818230
TGTGCCT252.3483153E-9229.998184
ATCCGGC600.0229.998187
AGTGCCA151.9525069E-5229.998184
Produced by FastQC (version 0.11.9)