FastQCFastQC Report
Wed 30 Nov 2022
4061-JW07trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW07trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216726
Sequences flagged as poor quality0
Sequence length231
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA14079764.96544023328997No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA135476.250749794671613No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA35301.6287847327962495No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA24251.1189243560994067No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA16290.7516403200354365No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG10350.4775615293042828No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG6160.2842298570545297No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA6070.28007714810405765No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA5680.26208207598534555No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA4910.22655334385352932No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG4740.2187093380581933No Hit
TAGCCGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG4480.2067126233123852No Hit
TAGCCCTAAACGATGAGCGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG4390.2025599143619132No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA4050.1868719027712411No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT3980.1836420180319851No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA3900.17995072118712105No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA3700.17072247907496102No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG3520.16241706117401697No Hit
TAGCTGTAAACGATGGGCACTAGGTGTCAGGGGCTTCGACCCCCTTGGCG3520.16241706117401697No Hit
TTGCCTTAAACGATGTATACTTGACGTAGTGGGGCTCAACCCCGACTGTG3220.14857469800577686No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA3160.14580622537212887No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG3010.1388850437880088No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG2930.13519374694314482No Hit
TGGCTGTAAACGATGAGCACTAGGTGTCAGGGGCTTCGACCCCCTTGGCG2840.13104103799267278No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2810.12965680167584878No Hit
TAGCTGTAAACGATGTAGACTTGGCGTAGGTTGGGTAAAACCGATCTGTG2760.12734974114780875No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2750.12688832904220076No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2720.12550409272537674No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT2690.12411985640855275No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG2590.1195057353524727No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG2510.1158144385076087No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2380.10981608113470466No Hit
ACGCTGTAAACGTTGGGTACTTGGTGTTGGGGGTTTGAGTCCCTCAGTGC2310.10658619639544863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATC550.0224.99019
CCCTAAA5300.0224.99014
GGCCCTA550.0224.99012
AACACTC450.0224.99019
AACGTTG850.0224.990089
AGCGTTC152.1321192E-5224.990086
AACGTAT350.0224.99008225
CACGGTA152.1321192E-5224.990082
AACCATG152.1321192E-5224.990089
TAAACCA152.1321192E-5224.990087
CACTGTA350.0224.990082
AAACGAG152.1321192E-5224.990088
AAACGAC152.1321192E-5224.990088
AGACGAT152.1321192E-5224.990088
AAACAAT4650.0224.990088
ACTCGTG152.1321192E-5224.99008225
TTAACAA152.1321192E-5224.99008225
AGTTAGC350.0224.99008225
CGTAACC152.1321192E-5224.990085
CGTAAAA350.0224.990085
Produced by FastQC (version 0.11.9)