FastQCFastQC Report
Wed 30 Nov 2022
4061-JW07trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW07trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216726
Sequences flagged as poor quality0
Sequence length236
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA11872554.78115223831013No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA121565.608925555770881No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA110005.07553316168803No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA52662.4297961481317425No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA50492.3296697212148056No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT26051.201978535108847No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA16630.7673283316261086No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC7920.36543838764153813No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA5930.27361737862554564No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA4030.1859490785600251No Hit
CACCGGTGCTCAAAAGAGGGGCTCCTATTTCTAGGAGCTTACACCGGGAT3660.168876830652529No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA3360.15503446748428892No Hit
TCAGGCTCCCCGAAGGGTCGGTTACATCTCTGCTCCCTACCACCTGTATG3310.15272740695624892No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG3030.13980786799922484No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC2970.13703939536557683No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC2950.1361165711543608No Hit
GTTCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGACAT2880.1328866864151048No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC2860.13196386220388878No Hit
ACAAGCTCCCCGAAGGGTCGGTCACCTTTCGGTTTCCTACCACTTGTATG2700.12458126851416074No Hit
TACAGCGTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2620.12088997166929671No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA2590.1195057353524727No Hit
GCAACAGTCCCTTGCGGGAAAGCAACATTTCTGTTGCGGTCTATTGCATG2440.11258455376835268No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC2410.11120031745152867No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2330.10750902060666463No Hit
TACAGCTTCCCGAAGGACATCATCTTTCAATGAGTTTAGCTGCAATATCA2330.10750902060666463No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC2280.10520196007862462No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG2210.1019720753393686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCATT400.0230.047796
GTCTGGT100.0017814538230.047797
CAAGTAG400.0230.047799
GGTCCAG100.0017814538230.047795
ATTCGGT100.0017814538230.047797
CAATTGC100.0017814538230.047799
TGTCTGG100.0017814538230.047796
GGTATTG202.1338383E-7230.047798
TTCGAAA202.1338383E-7230.047799
ATCTCGA252.3464963E-9230.047787
TCCTGAA151.9511666E-5230.047788
GGTCCGA151.9511666E-5230.047786
GGCCCGA151.9511666E-5230.047786
CAAGGCG252.3464963E-9230.047785
GATTCTG252.3464963E-9230.047785
GTACGCC151.9511666E-5230.047785
AGCAACC100.0017826852229.994724
GTACAGG100.0017826852229.994721
GTGGGTA100.0017826852229.99472230
ATAGCTC100.0017826852229.994722
Produced by FastQC (version 0.11.9)