FastQCFastQC Report
Wed 30 Nov 2022
4061-JW08trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW08trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149974
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA6088040.59370290850414No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG3368722.46189339485511No Hit
TAGCCGTAAACGATGGATACTAGGCGTGGCTTGTATCGACCCGAGCCGTG44362.9578460266446185No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC30502.033685838878739No Hit
TAGCCGTAAACGATGGATACTAGGCGTAGCTCGTATCGACCCGAGCTGTG19531.3022257191246482No Hit
ACGCCGTAAATTATGAGTGCTATCACTAAGATCACTCGATCTTGGTGAGA9930.6621147665595369No Hit
TAGCCGTAAACGATGGATACTAGATGTTGTGTGTATCAACCCATACAGTA8170.5447610919225999No Hit
ACGCCGTAAACGTTGGGCACTAGGTGTGGGTCTCAACCAACGAGATCCGC7980.5320922293197488No Hit
TAGCCGTAAACAATGGATACTAGATGTTGACCAGATCGACCTGTGCAGTA7380.49208529478442936No Hit
TAGCTGTAAACGATGGGCACTAGCTTGGGGTCTCCCTGTGTGATCCCAGG5770.3847333537813221No Hit
TAGCCGTAAACAATGGATACTAGATGTTGCACAGATCGACCTGTGCAGTA5750.38339978929681146No Hit
TAGCCGTAAACGATGGACACTAGGTGTTGGCCGTATCGACCCGGTCAGTG5210.34739354821502394No Hit
ACGCCCTAAACTATGGATACTCGACATACGCGATAAACTGTGTGTGTCTG5060.33739181458119405No Hit
ACACCGTAAACGATGAGCACTCGGTGCTGCGGGTGTCGACCCCTGCAGTG4410.294050968834598No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTGAGTATCGACCCTCACAGTG4130.2753810660514489No Hit
TAGCTGTAAACGATGAGCACTTGTTTGGAGGGTCCTCCATAGCCTTCCAG3810.2540440342992785No Hit
ACGCCGTAAACGATGGGTGCTAGGTGCTGCGGGCTTTGACCCCTGCGGTG3710.2473762118767253No Hit
ACGCCTTAAACGATGAATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC3520.23470734927387413No Hit
TAGCCGTAAACGATGGGTACTAGGCAAGGGGGCCACCTATGTGCCTCTTT2750.18336511662021415No Hit
ACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTG2600.17336338298638432No Hit
ACACTGTAAACGTTGATGACTCGATGTTGGCGATACACAGCCAGCGTCCA2550.1700294717751077No Hit
ACGCCTTAAACGATGGATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC2500.16669556056383106No Hit
ACGCTGTAAACGATGAGTGCTAGGCGTCGGCACGCTTGCGTGTCGGTGCC2430.1620280848680438No Hit
TAGCCGTAAACGATGGGCACTAGGCAAGGGAGGTACCTATGTCCTTCCTT2280.15202635123421393No Hit
ACGCTGTCAATGCTGAGTGCTGATACTTGGGAAAAATTCTTTGGTATTAA2250.15002600450744796No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTTCAGTA2160.14402496432715003No Hit
TAGCCGTAAACGATGGATACTAAGTACTGTGCTCTTCTAAAGCTCAGTAC2050.13669035966234147No Hit
TAGCCGTAAACGATGAGCACTGGACCGGAGCTCTGCACAGGGTTTCGGTC2030.13535679517783084No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGGGCAGTA2000.13335644845106487No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGGCCGTATCGACCCGGTCAGTG1950.13002253723978824No Hit
TAGCCGTAAACAATGGATACTAGGTGTAGCCTGTATCGACCCGGGCTGTG1920.12802219051302224No Hit
CAGCTGTAAACTATGCAAACTCAGTGATGCATTGGCTTGTGGCCAATGCA1870.12468827930174563No Hit
ACGCTGTAAACGATGAACACTAGGTGTCGTGGGTATTGACCCCCGCGGTG1820.12135436809046901No Hit
TAGCCGTAAACGATGGATACTAAATGTTGCGTGTATTGATCCACGCAGTG1770.1180204568791924No Hit
ACGCCCTAAACTATGGATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC1730.1153533279101711No Hit
ACGCCGTAAACGATGGGTGCTAGGTGTCGCGGGCTTTGACCCCTGCGGTG1560.10401802979183059No Hit
ACACTGTAAACGTTGATAACTCGCTGTTGGCGATACACAGCCAGCGGCCA1500.10001733633829862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCT2950.0225.000021
CGCCCTA3000.0225.000022
AACAATC303.092282E-11225.09
TGTCAAT202.3809844E-7225.05
TCTCTTA100.0019032016225.0225
CGACGTT100.0019032016225.02
GGGTCGT100.0019032016225.0225
CCCTAAA5000.0225.04
ACACCGT800.0225.01
ACCGATG100.0019032016225.09
CGACCTA100.0019032016225.02
TAAACGG550.0225.07
CACTGTA1200.0225.02
CCTAAAC5100.0225.05
TAGCCGG152.1304395E-5225.01
TAGCCGC100.0019032016225.01
TAGCCGA100.0019032016225.01
GTCAGCT100.0019032016225.0225
AACGCTG252.6757334E-9225.09
GCCCTAA5000.0225.03
Produced by FastQC (version 0.11.9)