FastQCFastQC Report
Wed 30 Nov 2022
4061-JW08trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW08trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149974
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT5886039.24680277914839No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT2068913.795057810020403No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGAAGGTTCGCGACATGT112577.505967701068185No Hit
GTTCGCGCTCCCGAAGGCACTCTCCCCTTTCAAGGAGATTCGCGACATGT45573.038526677957513No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC31982.132369610732527No Hit
CTTGGCGCTCCCTAAGGCACTCTCCTCTTTCAAAGAGATTCGCCAGATGT17561.1708696174003492No Hit
ATGATCTCGCGTGATCTCTACCTAAGAGCGCGGACAGACCATAATTCAAG9710.6474455572299198No Hit
GTGCGTGTTCCTAACGGCACTTCTTTCTTTCCAAAGAATTCACGCCATGT7960.530758664835238No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGCAATTCGTATCATGT7070.4714150452745143No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT6690.4460773200688119No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGACATTCGTATCATGT6200.41340499019830107No Hit
GCAGGATGGTATTGCTACCTCGTTAGGCTTTCACCCTTCTACTACCTGCA5610.3740648379052369No Hit
CTGGCAGTGTATTGCTACAAAGTGAGCTTTCACCCACGGTCTCCCAGCAT5150.34339285476149195No Hit
GTATCCGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT4910.3273900809473642No Hit
GTTCGTGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCACGACATGT3970.26471255017536377No Hit
GCAGGTTACACCCGAAGGCAATCCGCAGACTTTCATCCGCTTAATCCCTG3930.26204542120634244No Hit
GTACGACCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA3820.2547108165415339No Hit
CCGCAGGTTCTCCGAAGAGCACCCCGACCTTTCGACCAGGTTCCTGCGTT3540.2360409137583848No Hit
ACAGCAGCCCCCGAAGGGGAAACCTGGTTTCCCAGGCGGTCTCCTGCAGT3430.22870630909357623No Hit
GCACGTTGTACCCGAAGGCCTGGCTCGGCTTTCACCGAGTTAATCCGTGC3300.220038139944257No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGACGGTTCGCGACATGT3190.21270353527944844No Hit
GCATGTTCCGACTCCGAAGAGCTGTCATCCCGCTTTCACGGGACTAATCC3010.2007014549188526No Hit
GTATACGGTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2410.16069452038353316No Hit
CCACTAGCTCCCTTGCGGGCACATCAGACTTTCATCCGACTTCTAATGAC2330.15536026244549056No Hit
GTTCACTTGCCCGAAAGCTACCGATTAAAAATCGACAATCTCCTTTCGGA2300.15335991571872457No Hit
AAAGCTAGTTTTTTCAATTTTACGCTATGAACTTATTGAAAAATTTATAT2280.15202635123421393No Hit
GTTCGCGCTCCCGAAGGCACTCCCTCCTTTCAAAGGGATTCGCGACATGT2110.1406910531158734No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGCAGGTTCGCGACATGT2100.1400242708736181No Hit
CTCCTTGTCCTTGCGGAACGATACATCTCTGTACCCATCAAGGGCATTCT2050.13669035966234147No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCTCTCC2030.13535679517783084No Hit
CACTCTGCTCCCGAAGGAGAAGCCCTATCTCTAGGGTTTTCAGAGGATGT2030.13535679517783084No Hit
TTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT1740.11602011015242643No Hit
GCAGATTCCGACTCCGAAGAGCTGTCATCCCGCTTTCACGGGACTAATTC1680.11201941669889447No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGACGGTTCGCGACATGT1570.1046848120340859No Hit
CCAGCACCTTCGAAAGCTTCTCTGGTTTCCCAGAGCGTGCAGCTGGATTT1500.10001733633829862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCG350.0230.06
CGTATTG252.3464963E-9230.09
ATGATCT1000.0230.01
TAAAGGC252.3464963E-9230.02
ATGGCGG350.0230.01
TGTGGAC700.0230.0230
TCCCTAA1550.0230.09
TGGCAGT500.0230.02
CAGTGTA500.0230.05
GTATCCG1050.0230.01
CACGTTG500.0230.02
ATACGCT350.0230.03
GCAGATG252.3464963E-9230.01
AGCAGCC252.3464963E-9230.03
AGTGTAT500.0230.06
TCCGGAG100.0017820166229.999989
CAGGTTA550.0229.999982
CAGAATT100.0017820166229.999982
CTCACGG100.0017820166229.999981
ACGCCAG4250.0229.999985
Produced by FastQC (version 0.11.9)