FastQCFastQC Report
Wed 30 Nov 2022
4061-JW09trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW09trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170074
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA9735957.24508155273587No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA106516.262568058609781No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG26851.5787245551936218No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA22861.3441207944776978No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA21451.2612157061044016No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG13130.7720168867669367No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG9390.5521126098051437No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG7950.46744358338135167No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT7140.41981725601796865No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG6310.3710149699542552No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG5910.3474957959476463No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG5900.3469078165974811No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT5030.2957536131331068No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG5010.29457765443277634No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC4260.25047920317038463No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG4180.24577536836906289No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG4050.238131636816915No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA4010.2357797194162541No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3610.21226054540964523No Hit
TAGCCGTAAACTATGGATACTGGATCTTGCATATATTTGCTTGGTCGAAG3470.20402883450733209No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3450.20285287580700165No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA3400.19991297905617555No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA3180.18697743335254066No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3080.18109763985088842No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG3060.17992168115055798No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3010.1769817843997319No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2810.16522219739642743No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2760.1622823006456013No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG2720.15993038324494044No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA2700.15875442454460997No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2660.1564025071439491No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2590.15228665169279254No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2530.1487587755918012No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2460.14464292014064467No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2350.1381751472888272No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA2320.13641120923833155No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2270.13347131248750546No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG2260.13288333313734021No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2240.13170737443700978No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA2230.13111939508684456No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2100.12347566353469668No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2100.12347566353469668No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG2080.12229970483436621No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2000.11759587003304445No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG1970.11583193198254878No Hit
GGAAAGGCGTTCATGGCGCAGATGTCCGAGACGTTGACGCTGATCGCCTT1950.11465597328221833No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1940.1140679939320531No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1870.10995213848089655No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC1750.10289638627891388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGT1900.0225.000028
CAGACGG350.0225.00002225
TCGCCGT100.0019034253225.01
CAGAATC100.0019034253225.0225
AACAATC152.1309399E-5225.09
TTTACTA152.1309399E-5225.0225
CACGGTA252.6775524E-9225.02
CGACGTA100.0019034253225.02
CTATAAA100.0019034253225.04
GCTATAA100.0019034253225.03
TAGCCCT850.0225.01
AGACGAT152.1309399E-5225.08
GTAGACG152.1309399E-5225.06
TACGACG152.1309399E-5225.0225
CTTGCAC100.0019034253225.0225
GTCGTAA100.0019034253225.03
CGAATTC100.0019034253225.0225
AACGATA2050.0225.09
CAGGAAA100.0019034253225.04
TTAACAA100.0019034253225.0225
Produced by FastQC (version 0.11.9)