FastQCFastQC Report
Wed 30 Nov 2022
4061-JW09trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW09trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170074
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA6935040.776367933958156No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA165779.74693368768889No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA121117.121017909851006No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA83334.899631924926797No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC19091.1224525794654092No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC15410.9060761786046074No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA13580.7984759575243718No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA5610.3298564154426897No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG5340.31398097298822863No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5330.31339299363806344No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA4950.291049778331785No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4810.28281806742947185No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC4760.2798781706786458No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA4420.2598868727730282No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT4290.2522431412208803No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC3910.22989992591460187No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT3810.22402013241294966No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA3810.22402013241294966No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT3200.1881533920528711No Hit
GACGACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2960.17404188764890577No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2870.16875007349741877No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA2780.16345825934593178No Hit
ACAGGTTTCTCCAAAGGAGAATCACTATCCCTTTCAGGTAGCTATGCCTG2640.15522654844361866No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG2570.1511106929924621No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2470.1452308994908099No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA2380.1399390853393229No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG2290.13464727118783587No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2290.13464727118783587No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2210.1299434363865141No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2200.12935545703634888No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2140.12582758093535756No Hit
GAGGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCCACATG2110.12406364288486188No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2070.12171172548420099No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG2050.12053576678387055No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATATAACCGTCGCCCC2000.11759587003304445No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT1960.11524395263238356No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC1960.11524395263238356No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1890.111128097181227No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA1830.10760022108023566No Hit
GACGCCTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1800.10583628302974No Hit
GCAGCAAGGCGATCAGCGTCAACGTCTCGGACATCTGCGCCATGAACGCC1730.10172042757858343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA100.0017822257230.000028
TAGAGCC100.0017822257230.000022
CTCACTG100.0017822257230.000021
TTGGACC100.0017822257230.00002230
AGATAAC100.0017822257230.000026
ATCAGGT100.0017822257230.000027
ACTCTGT100.0017822257230.000022
TTGTCGA100.0017822257230.000025
GCGCCTA100.0017822257230.000025
ATTCGGT100.0017822257230.000027
ACCTCTG100.0017822257230.000022
GTGGACG1600.0230.00002230
AACATTG202.1347114E-7230.00002230
TTAAGTA100.0017822257230.000028
GTAGAGC100.0017822257230.000021
CGCTATA100.0017822257230.000028
GTATGCC100.0017822257230.000025
GGTATTG202.1347114E-7230.000028
CTTGAAG100.0017822257230.000029
GAAAAAT100.0017822257230.000022
Produced by FastQC (version 0.11.9)