FastQCFastQC Report
Wed 30 Nov 2022
4061-JW10trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW10trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117772
Sequences flagged as poor quality0
Sequence length231
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA3016125.60965254899297No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA113959.675474645926027No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA83187.062799307135821No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG26702.2670923479265017No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG20891.773766260231634No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA19561.6608361919641341No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG12971.1012804401725367No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG11420.9696702102367286No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG10360.8796657949257889No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT10350.8788166966681384No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC8090.6869204904391536No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG8080.6860713921815033No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT5610.4763441225418606No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG5580.4737968277689094No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG5420.4602112556465034No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG4730.4016234758686275No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG4160.35322487518255613No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG4000.3396393030601501No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG3890.330299222225996No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG3840.32605373093774415No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG3730.31671365010359No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG3660.3107699623000374No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG3610.3065244710117855No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG3550.30142988146588323No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG3540.30058078320823284No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG3400.2886934076011276No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3380.2869952110858269No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG3270.2776551302516727No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3230.27425873722107125No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGGGGTATTGATCCCTTCGGTG3100.26322045987161635No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG3050.2589749685833645No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3020.2564276738104133No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC2880.24454029820330808No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG2790.2368984138844547No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG2750.23350202085385321No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2740.23265292259620285No Hit
ACGCTGTAAACTTTGGGCACTAGGTATTGGAGGTCTCAACCCCTTCAGTG2720.2309547260809021No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2670.2267092347926502No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2660.22586013653499984No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG2650.22501103827734945No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2630.22331284176204869No Hit
ACGCCGTAAACGATGATTACTCGTTGTTGGCGATATATTGTCAGCGACTG2600.22076554698909756No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2470.2097272696396427No Hit
TGGCCGTAAACGATGAGCACTAGGTGTGGGAGGTATCGACTCCTCCTGTG2400.20378358183609008No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2380.20208538532078932No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG2280.19359440274428558No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2280.19359440274428558No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG2250.19104710797133445No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT2200.18680161668308257No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA2180.1851034201677818No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2140.18170702713718032No Hit
GTGCCGTAAACGATGCAGACCAGGTGTGGCGGGCGCAAGCCTGCTGTGCC1960.16642325849947356No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGAGGTATTGATCCCTTCGGTG1920.16302686546887207No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG1900.1613286689535713No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA1890.16047957069592092No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA1860.1579322759229698No Hit
TAGCCGTAAACGATGGATACTAGGTGTTAGAGGTATCGACCCCTCTAGTG1830.1553849811500187No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG1820.1545358828923683No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCATCGGTG1810.15368678463471794No Hit
ACGCCCTAAACGATGGGCACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1760.14944129334646605No Hit
ACGCTGTAAACGATGAATACTAGGTGTTGGGTTTTAAACTCAGTGCCGCA1710.1451958020582142No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGATCAGTGTCGCA1700.1443467038005638No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG1650.14010121251231192No Hit
ACGCCGTAAACGATGTCAACTAGGTGTTGGGATGGTTAATCGTCTCATTG1580.1341575247087593No Hit
ACGCCGTAAACGATGAGTACTAGGTGTCGGGGGTTACCCCCCTCGGTGCC1560.13245932819345854No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1550.13161022993580818No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1550.13161022993580818No Hit
ACGCTGTAAACGATGAATACTAGGTGTTGGGTTTTTAACTCAGTGCCGCA1520.12906293516285705No Hit
TAGCTGTAAACGATGTAGACTTGGCGTAGGTTGGGTAAAACCGATCTGTG1470.12481744387460518No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1470.12481744387460518No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA1400.11887375607105254No Hit
TAGCTGTAAACGATGTGGACTGGGTGTCGGTGGGGTAAAAGCCATCGGTG1380.11717555955575178No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA1380.11717555955575178No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG1310.11123187175219916No Hit
TAGCCGTAAACGATGTGAACTGGGTGTCGGTGGGGTAAAAGCCATCGGCG1300.11038277349454878No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1300.11038277349454878No Hit
TAGCCGTAAACGATGTAGACTTGGTGTTGGAGGGGTAAAAGCCTTCAGTG1290.10953367523689841No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1270.10783547872159766No Hit
ACGCCGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCCCTCAGTG1230.10443908569099616No Hit
TAGCCGTAAACGATGGACACTAGGTGTGGGGAGTTATCGACGCTCTCTGT1200.10189179091804504No Hit
ATGCCGTAAACGATGTGCACTAGGTGTAGGGGTGTCACAGCCGCTGTACC1200.10189179091804504No Hit
ACGCTGTAAACGATGAGTGCTTGGTGTAGCGGGTATCGACCCCTGCTGTG1180.10019359440274428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG350.0225.000029
GGCCCTA500.0225.000022
GCCATAA252.6720954E-9225.000023
GTAACCG252.6720954E-9225.000026
TTTAGCA100.0019026842225.0225
CAAACAA100.0019026842225.0225
GTCTGTT100.0019026842225.0225
CATGCGA100.0019026842225.04
CTGAGTC100.0019026842225.0225
TTGTCAA100.0019026842225.0225
CCATGCG100.0019026842225.03
TGTTGTA100.0019026842225.0225
AGACGAT100.0019026842225.08
GCACAGT100.0019026842225.01
CCGTAAC303.092282E-11225.04
TGCGATC100.0019026842225.06
TACGACG152.1292813E-5225.0225
CCATAAA202.3788198E-7225.04
CCCCATG100.0019026842225.01
CATAAAC202.3788198E-7225.05
Produced by FastQC (version 0.11.9)