FastQCFastQC Report
Wed 30 Nov 2022
4061-JW10trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW10trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117772
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1809215.361885677410589No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1711414.531467581428522No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1652314.029650511157152No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC19681.6710253710559386No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC14641.2430798492001496No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT12151.031654383045206No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC10280.8728730088645859No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA8690.7378663858981761No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT6370.5408755901232891No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA6280.5332337058044357No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5980.5077607580749244No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC5950.5052134633019733No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA5900.5009679720137215No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC5860.4975715789831199No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT4370.371055938593214No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT4140.3515266786672554No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA4100.3481302856366539No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3920.33284651699894713No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT3890.330299222225996No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG3810.323506436164793No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT3630.3082226675270862No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC3600.3056753727541351No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC3580.30397717623883436No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA3550.30142988146588323No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA3510.2980334884352817No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT3450.29293889888937946No Hit
ACACCTGCTCCCGAAGGAGGGACTCCTATTTCTAAGAGTTTACAGGTGCG3370.2861461128281765No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT3230.27425873722107125No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG3210.27256054070577046No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA3150.2674659511598682No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC3010.255578575552763No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2980.25303128077981185No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA2940.24963488774921036No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG2870.2436911999456577No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG2660.22586013653499984No Hit
GCAGCAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCACTGCAGTTC2570.21821825221614646No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2500.21227456441259385No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG2450.20802907312434193No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG2400.20378358183609008No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2340.1986889922901878No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2290.19444350100193594No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG2270.19274530448663518No Hit
CTCCCTGTCCCTTGCGGGAAAGCCTGCTTTCACAGGATTTCAGGGGATGG2260.19189620622898482No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT2210.18765071494073296No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC2200.18680161668308257No Hit
CTCCAGGTTCTCTCTAATGAAAGCACTCTCCTGTTTCCAGGAGATTCCCG2190.1859525184254322No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2000.16981965153007506No Hit
CACCCTGTCCCGAAGGACTTCGCCCATCTCTGGGTTATGCAGGGGATGTC2000.16981965153007506No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA1990.1689705532724247No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1980.1681214550147743No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1820.1545358828923683No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1810.15368678463471794No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1800.15283768637706754No Hit
GTGACAGCTCCTTGCGGAGAGAGTGGCTTTCACCACCTGTCTGCCCCTCT1790.15198858811941718No Hit
CTTGGAGCTGTATGTTACCATACAGGGGTACCACTTTCATGGACTTACAC1660.14095031076996228No Hit
ATAGGCTCCCCGAAGGGTCGGTCACCTCTCGGCTTCCTACCACCTATATG1620.1375539177393608No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACTTGCATG1600.13585572122406006No Hit
ATACATGTCCGAAGAAGGGTGTATCTCTACACCTGTCATGTACAGTTCAA1520.12906293516285705No Hit
ATTAGCTCCTATATTGCTATAGGTCGGTCCCCTTTCAGTTTCCTACCACT1520.12906293516285705No Hit
ATAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTATATG1470.12481744387460518No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC1460.1239683456169548No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1450.12311924735930442No Hit
CCACGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACGTGCATG1440.12227014910165404No Hit
CAGCTTGTCTGGTAAGGTCGTCAGCCTGACCTTCATTCTGCTGTCGCCCC1440.12227014910165404No Hit
GCAGGCTCCTTTCGGCCACGGCGTTTCCGCCGCTTCACCTGCATGTCAAG1430.12142105084400366No Hit
GTGGACGCGCCCTACTTTCATAGAGCTGACCTCACCCGAAGGCAAGGCTC1410.11972285432870292No Hit
CTCCTGGCTCTCTTGCGAGCACCCCCCCGTTTCCGGGAGGTTCCAGGGAT1400.11887375607105254No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCGCCCC1380.11717555955575178No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCACTTCCCTACCACTTGCATG1370.11632646129810142No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC1370.11632646129810142No Hit
CACCGGTCCAGCCGAACTGACCCACCTGTTTCCAGGCAGTACGACCGGGA1350.11462826478280067No Hit
TAACCTGCCAATTGCTTGGAAATACCCTTTCAGGTACGGTCACGCTACAT1330.11293006826749992No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1330.11293006826749992No Hit
ACAAGCTCCCCGAAGGGTCGGTCACCTTTCGGTTTCCTACCACTTGTATG1330.11293006826749992No Hit
GATTGCTCCCCGAAGGGTCGCTCCGCTTTCGCTTCGCTACCACAACCATG1320.11208097000984954No Hit
GCATGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACATGCATG1310.11123187175219916No Hit
CAGCTTGTCAAGCAAGGTCATCAACCTGGCAATCATACTGCTGTCGCTCC1290.10953367523689841No Hit
TCAGGCTCCCCGAAGGGTCGGTCCACTTTCGTTTCCCTACTACCTGTATG1280.10868457697924803No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA1260.10698638046394728No Hit
ACAGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCTGTATGTC1260.10698638046394728No Hit
CAGCGATTCAGGTAAGACCTTCAGCCTGACCTACATATTGCTGTCGCCCC1240.10528818394864654No Hit
CTCTCTGTCCCGAAGGATTTCCTCAGTTACGAGTAATGCAGAGGATGTCA1240.10528818394864654No Hit
CACCGATCCAGCCGAGCTGACCCGCCTGTTTCCAGGCAGTACGATCGGGA1230.10443908569099616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAG1000.0230.000029
AGGACAG500.0230.000021
TACGACT550.0230.000021
CTTGTCT700.0230.04
GTTGACC350.0230.0230
CGGAAAA700.0230.01
AGTCCCG151.950528E-5230.06
GCAAGTT151.950528E-5230.01
CGTATTG750.0230.08
GTCTGGT1050.0230.07
CGCGGCT151.950528E-5230.03
TTGTCTG650.0230.05
TGACGAC350.0230.02
TATTGCC151.950528E-5230.03
CGCTCTA151.950528E-5230.08
AGTTCTT302.5465852E-11230.06
TGGGGCT151.950528E-5230.03
TCCCGAG151.950528E-5230.08
GACGCGC350.0230.04
AGCTCGT750.0230.02
Produced by FastQC (version 0.11.9)