FastQCFastQC Report
Wed 30 Nov 2022
4061-JW11trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW11trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143846
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA5814540.421700985776454No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA108737.558778137730629No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA62154.320592856249044No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA38092.6479707464927773No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG32512.260055893107907No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG13760.9565785631856292No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT11800.8203217329644203No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG10280.7146531707520543No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG9760.6785033994688765No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG9140.6354017490927798No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT8640.6006423536281857No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG7840.5450273208848352No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC7660.5325139385175812No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG7620.5297331868804138No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG6550.4553480805861825No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG5810.4039041752985832No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG4400.3058826800884279No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG4300.2989308009955091No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3760.2613906538937475No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC3730.25930509016587183No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG3560.24748689570790985No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA3460.24053501661499105No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG3400.23636388915923975No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA3100.21550825188048328No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2890.2009093057853538No Hit
ACGCCCTAAACGATGGGTACTTGGTGTCGCGGGTATCGACTCCCGCGGTG2840.19743336623889435No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2660.18491998387164052No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2520.17518735314155418No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA2500.1737969773229704No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2470.17171141359509476No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG2470.17171141359509476No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG2400.1668450982300516No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT2400.1668450982300516No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2350.16336915868359217No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2310.16058840704642466No Hit
TAGCGGTAAACGATGGAAACTAGGTGTTGCCTGTATCGACCCGGGCAGTG2300.15989321913713275No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2260.15711246749996524No Hit
ACGCCCTAAACGATGCGAACTGGACGTTGGGCTCAATTGAGAGTTCAGTG2250.15641727959067334No Hit
ACGCCCTAAACGATGGGCACTAGGTGTGGGAGGTATCGACCCCTTCCGTG2250.15641727959067334No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA2190.15224615213492207No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG2110.14668464886058702No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC2110.14668464886058702No Hit
ACGCCGTAAACGATGAGAACTAAGTGTTGGCGCAAGTCAGTGCTGAAGTT2090.14529427304200324No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2080.14459908513271136No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2030.14112314558625197No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2030.14112314558625197No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA2000.1390375818583763No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT1990.13834239394908443No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1960.1362568302212088No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1870.13000013903758184No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG1830.12721938740041433No Hit
TAGCAGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAATCCGTCGGTG1820.12652419949112245No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG1820.12652419949112245No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG1790.1244386357632468No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG1760.12235307203537116No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA1740.12096269621678739No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT1680.11679156876103611No Hit
ACGCTGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1670.11609638085174423No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT1610.11192525339599292No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG1600.11123006548670106No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGGGGTATTGATCCCTTCGGTG1600.11123006548670106No Hit
ACGCGGTAAACGATGGGCACTAGGTGTGGGGGGTATCCACTCCTCCCGTG1580.10983968966811729No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG1580.10983968966811729No Hit
TGGCCGTAAACGATGAGCACTAGGTGTGGGAGGTATCGACTCCTCCTGTG1490.10358299848449036No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG1490.10358299848449036No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG1470.10219262266590659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA5750.0225.000023
TCGCCGT152.1302583E-5225.01
AACGTTG2550.0225.09
AGCGTTC100.0019031208225.06
AACAATG400.0225.09
CACGGTA303.092282E-11225.02
TAGCGGT252.6739144E-9225.01
GCTATAA100.0019031208225.03
TAAACCA100.0019031208225.07
TAGCCCT500.0225.01
AAACGTT2550.0225.08
AGACGAT100.0019031208225.08
CGGATTT152.1302583E-5225.0225
CGTAGAC100.0019031208225.05
AAACAAT450.0225.08
CGTACAC100.0019031208225.05
CGTAACC252.6739144E-9225.05
CGTAAAT252.6739144E-9225.05
GACGATG100.0019031208225.09
GTCCACG100.0019031208225.03
Produced by FastQC (version 0.11.9)