FastQCFastQC Report
Wed 30 Nov 2022
4061-JW11trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW11trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143846
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA4385930.490246513632634No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1452010.094128442918121No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA123988.618939699400748No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA48983.4050303797116364No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC19711.3702153692142987No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC11920.8286639878759229No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC8940.6214979909069421No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA7910.5498936362498783No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT6880.4782892815928146No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG6140.4268453763052153No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA6060.4212838730308802No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT6040.4198934972122965No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA5880.40877049066362636No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5020.34898433046452454No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC4590.31909125036497366No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC4440.3086634317255954No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3850.2676473450773744No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT3470.24123020452428293No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA3340.23219276170348846No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA3200.22246013097340211No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2990.20786118487827263No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2850.19812855414818625No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA2840.19743336623889435No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2760.19187186296455933No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA2660.18491998387164052No Hit
GCAGCAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCACTGCAGTTC2640.18352960805305674No Hit
ATCCATTGCCCGAAGGAGAGCGCATCTCTGCGCCTGTCAATGGTATGTCA2620.18213923223447298No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2530.17588254105084605No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2510.17449216523226227No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2330.1619787828650084No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT2300.15989321913713275No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT2290.15919803122784087No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2290.15919803122784087No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC2230.1550269037720896No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG2080.14459908513271136No Hit
CTCACGGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCCGTGGATGT2080.14459908513271136No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2000.1390375818583763No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1950.13556164231191692No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1870.13000013903758184No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1840.1279145753097062No Hit
CTCCTGGCTCTCTTGCGAGCACCCCCCCGTTTCCGGGAGGTTCCAGGGAT1730.12026750830749552No Hit
ATTAGCTCCTATATTGCTATAGGTCGGTCCCCTTTCAGTTTCCTACCACT1730.12026750830749552No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG1700.11818194457961988No Hit
ACACCTGCTCCCGAAGGAGGGACTCCTATTTCTAAGAGTTTACAGGTGCG1690.11748675667032799No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1690.11748675667032799No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGTTTCCTACCACCTGTATG1670.11609638085174423No Hit
CTTCTGGTTCCCTAAGGCACTCTCTCGTTTCCAAGAGATTCCAGAGATGT1640.1140108171238686No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT1550.10775412594024164No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT1510.10497337430307413No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1460.10149743475661471No Hit
CTTACGGCTCCCCGAAAGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT1460.10149743475661471No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT1450.10080224684732282No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA1440.10010705893803094No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1440.10010705893803094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGACG850.0230.00002230
TGACAGC1100.0230.000022
TAGTCAA1100.0230.000025
GTGCACG151.9514231E-5230.0230
TTAGGGG151.9514231E-5230.0230
CAGCGCT252.3446773E-9230.01
CAGCGAT3550.0230.01
TGGAGCT151.9514231E-5230.03
AATCCGG1550.0230.06
AAATCAG650.0230.05
AATCAGG650.0230.06
TGCCATT252.3446773E-9230.06
GTCTGGT450.0230.07
ATAGGCT450.0230.01
TAAAGCT350.0230.01
CGAAAAA151.9514231E-5230.01
TCCTGTA302.5465852E-11230.07
AGCCCTC252.3446773E-9230.05
CAGCCGA2600.0230.09
CATTGCC500.0230.04
Produced by FastQC (version 0.11.9)