FastQCFastQC Report
Wed 30 Nov 2022
4061-JW12trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW12trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74630
Sequences flagged as poor quality0
Sequence length231
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1741423.333779981240788No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA768310.294787618920004No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA41355.540667291973738No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA30574.096207959265711No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG20612.7616240117915045No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG12651.695028808790031No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG7020.9406404930992898No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT6340.8495243199785609No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG6110.8187056143641966No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG6080.8146857831971057No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC4690.6284336057885569No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG4060.5440171512796462No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3750.5024788958863727No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT3490.4676403591049176No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG3360.45022109071419003No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG3320.4448613158247353No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3110.4167224976550985No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC3000.40198311670909825No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2830.379204073428916No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2490.3336459868685515No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2260.30282728125418734No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC2220.2974675063647327No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT2120.28406806914109606No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG1980.26530885702800483No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1910.2559292509714592No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1840.24654964491491355No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1840.24654964491491355No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT1820.24386975747018624No Hit
ACGCCGTAAACGATGAGCACTAAGTGTCGGGCGAATGCTCGGCGCTGCAG1770.23717003885836796No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1760.2358300951360043No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1730.2318102639689133No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1650.221090714190004No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG1640.21975077046764036No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG1630.2184108267452767No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG1610.21573093930054937No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA1590.21305105185582207No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG1510.20233150207691278No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG1440.19295189602036714No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG1390.18625217740854885No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG1370.18357228996382152No Hit
ACAGCTGTAAACGATGTGCACTAGATGTGGGGAAGTGTTGACCTTTCCTG1340.17955245879673054No Hit
ACGCCGTAAACGATGATTACTCGTTGTTGGCGATATATTGTCAGCGACTG1320.1768725713520032No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG1310.17553262762963956No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG1260.16883290901782125No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA1230.16481307785073027No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA1220.1634731341283666No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT1200.16079324668363928No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG1190.15945330296127563No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1150.15409352807182097No Hit
TGGCCGTAAACGATGAGCACTAGGTGTGGGAGGTATCGACTCCTCCTGTG1140.15275358434945732No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1140.15275358434945732No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGAA1110.14873375318236634No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG1070.1433739782929117No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG1040.13935414712582073No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG1020.1366742596810934No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGGGGTATTGATCCCTTCGGTG1010.13533431595872972No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG990.13265442851400241No Hit
ACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG950.12729465362454778No Hit
TAGCCGTAAACGATGGATACTAGGTGTTAGAGGTATCGACCCCTCTAGTG950.12729465362454778No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG950.12729465362454778No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGTGGGCGCCCA940.1259547099021841No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC930.12461476617982045No Hit
ACGCCGTAAACGATGAGAACTAAGTGTTGGCGCAAGTCAGTGCTGAAGTT920.12327482245745677No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTCCCGTG910.12193487873509312No Hit
TAGCTGTAAACGATGTGAACTTGGTGTCGGTGGGGTAAAAGCCATCGGTG910.12193487873509312No Hit
ACGCCGTAAACGATGGGCACTAGGTGTCGCGGGTATTGACCCCTGCGGTG890.1192549912903658No Hit
CAGCTGTAAACGATGCTAGCTAGGTGTTGGGTCGGCCACGCGCCGCCCCA890.1192549912903658No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA890.1192549912903658No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGACTTGTTCACTAGTGCCG870.11657510384563848No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGATCAGTGTCGCA860.11523516012327482No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGTTCAGTGTCGCA860.11523516012327482No Hit
CAGCTGTAAACGATGCAAACTAGGTGTTTGAACGGCCACGTGCCGTTCTA860.11523516012327482No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG850.11389521640091116No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT830.11121532895618383No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCATCGAG820.10987538523382018No Hit
ACGCCGTAAACGATGAGCACTAGGTGTCGGGGTCGCAAGACTTCGGTGCC800.10719549778909286No Hit
ACGCCGTAAACGATGTCAACTAGGTGTTGGGATGGTTAATCGTCTCATTG790.10585555406672921No Hit
ACGCCGTAAACGATGAATACTAGGTGTCAGCCCGCAGGGGTTGGTGCCGC780.10451561034436553No Hit
TGGCAGTAAACGATGTGAACTTGGCGTTGGTGGGATAAAACCTATCAGTG770.10317566662200188No Hit
ACGCTGTAAACGATGATTACTAAGTGTTGGAGCAATTCAGTGCTGCAGTT760.10183572289963821No Hit
ACGCCCTAAACGATGGGCACTAGGTGTGGGAGGTATCGACCCCTTCCGTG760.10183572289963821No Hit
TAGCCGTAAACGTTGGGTGCTAGGTGTGGGCGGATATCGACCCCGTCCGT750.10049577917727456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT350.0224.99331
AACAATG303.092282E-11224.99339
AACAATC152.1264166E-5224.99339
CACGGTA100.0019014605224.99332
ACACCGT152.1264166E-5224.99331
ACCGATG100.0019014605224.99339
ACCCTAA100.0019014605224.99333
GCCATAA303.092282E-11224.99333
TAGCAGT350.0224.99331
AAACGCT1300.0224.99338
AAACGAC152.1264166E-5224.99338
GCCCTAA2600.0224.99333
CCATAAA350.0224.99334
AACGATA2100.0224.99339
AAACAAT450.0224.99338
GAGCCGT152.1264166E-5224.99331
AGTTAGC100.0019014605224.9933225
CATAAAC350.0224.99335
CGTAAAT152.1264166E-5224.99335
ACCGTAA202.3733628E-7224.99333
Produced by FastQC (version 0.11.9)