FastQCFastQC Report
Wed 30 Nov 2022
4061-JW12trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW12trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74630
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1197116.040466300415382No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1009113.521372102371702No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA1007713.50261289025861No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA17332.322122470856224No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC17262.312742864799678No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC14891.9951762025994908No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA7521.0076376792174728No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC6700.8977622939836527No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC4970.6659520300147393No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA4580.6136942248425566No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4400.5895752378400108No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4180.5600964759480102No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT4020.5386573763901916No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA3340.44754120326946273No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGTTATGCCTA3330.446201259547099No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC3180.42610210371164414No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2730.36580463620527937No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2720.36446469248291574No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT2580.3457054803698245No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2220.2974675063647327No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT2030.2720085756398231No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC1990.2666488007503685No Hit
CTCCATTGTCCCGAAGGAAAGGCTTATCTCTAAGCCGGTCAATGGGATGT1800.2411898700254589No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA1730.2318102639689133No Hit
CAGCTAGTCAAGCAGAGTCTTCAACCCGGCTATCATTCAGCTGTCTTATC1730.2318102639689133No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA1710.22913037652418597No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC1650.221090714190004No Hit
CACCGGGTTCTTTATTGCTAAAGCACTTCTCTGTTTCCAGAAAATCCCCG1610.21573093930054937No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1540.20635133324400376No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG1540.20635133324400376No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1490.19965161463218548No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1450.19429183974273082No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCATTCTGCTGTCGCCCC1440.19295189602036714No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG1410.18893206485327615No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1340.17955245879673054No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATATTGCTGTCGTCCC1290.17285274018491226No Hit
CTCCCTGTCCCTTGCGGGAAAGCCTGCTTTCACAGGATTTCAGGGGATGG1260.16883290901782125No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG1260.16883290901782125No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1250.1674929652954576No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT1190.15945330296127563No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1190.15945330296127563No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA1180.15811335923891198No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA1180.15811335923891198No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1170.1567734155165483No Hit
ATACATGTCCGAAGAAGGGTGTATCTCTACACCTGTCATGTACAGTTCAA1130.15141364062709367No Hit
CTTCCTGCCCCGAAGGGCTTCCCCGGTTAAGGGTAATTCAGGAGATGTCA1120.15007369690473No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1110.14873375318236634No Hit
TACAGTTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAACTGCAATATCA1110.14873375318236634No Hit
GCAGCAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCACTGCAGTTC1100.1473938094600027No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1070.1433739782929117No Hit
TACACCTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA1060.14203403457054803No Hit
GCATGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACATGCATG1050.14069409084818438No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA1040.13935414712582073No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1030.13801420340345705No Hit
CACCGATCCAGCCGAGCTGACCCGCCTGTTTCCAGGCAGTACGATCGGGA1030.13801420340345705No Hit
CGGAAAATCAGGTAAAGTCATCAGCTTGACCTTCATATATCCGTCGCTCC1000.13399437223636607No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT990.13265442851400241No Hit
CACCCTGTCCCGAAGGACTTCGCCCATCTCTGGGTTATGCAGGGGATGTC980.13131448479163876No Hit
CAGCTCGTCTAGCAAGACCTTCAATCTGGCCTTCACTCTGCTGTCGATCC980.13131448479163876No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG970.1299745410692751No Hit
GCAGGCTCCTTTCGGCCACGGCGTTTCCGCCGCTTCACCTGCATGTCAAG960.12863459734691143No Hit
ATCCATTGCCCGAAGGAGAGCGCATCTCTGCGCCTGTCAATGGTATGTCA940.1259547099021841No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG910.12193487873509312No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT880.11791504756800215No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC880.11791504756800215No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCATACTGCTGTCGCTCC880.11791504756800215No Hit
GCTAACTCCCCGAAGGACCTCGCCTTTCAACGAGTTTAGTTAGCATATCA860.11523516012327482No Hit
CTTGGAGCTGTATGTTACCATACAGGGGTACCACTTTCATGGACTTACAC820.10987538523382018No Hit
GCTAACTCCCCGAAGGACCTCATCTTTCAATGAGTTTAGTTAGCATATCA810.10853544151145651No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATACAGCTGTCTTATC800.10719549778909286No Hit
GCAGGCTCCGAAGAGCGCCCGCTTTCACGGGTTTCACCCTGCATGTCAAA800.10719549778909286No Hit
CAGCGATTCAGGCAAAGTCTTCAACTTGGCCTACATATTGCTGTCGCCCC780.10451561034436553No Hit
CAGCTTGTCAAGCAAGGTCATCAACCTGGCAATCATACTGCTGTCGCTCC770.10317566662200188No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG760.10183572289963821No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG760.10183572289963821No Hit
ATAGGCTCCCCGAAGGGTCGGTCGCATCTCTGCTCCCTACCACCTATATG750.10049577917727456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG650.0230.142875
CATTGTC302.5465852E-11230.142874
CGCCTAT302.5465852E-11230.142876
TGTCTGG650.0230.142876
CCTGCCC600.0230.142874
AATCAGG1050.0230.142876
GTTCTCT151.942848E-5230.142877
GAGCTGT151.942848E-5230.142875
CTGATTT151.942848E-5230.142879
GTCTGGT650.0230.142877
CGGGCTC151.942848E-5230.142874
GCCAGCC151.942848E-5230.142877
CACCCGA151.942848E-5230.142879
TGCCATT151.942848E-5230.142876
GCCATTG151.942848E-5230.142877
CTTGTCT650.0230.142874
CTGCCAA151.942848E-5230.142875
GCGTATT151.942848E-5230.142877
ATTCTGG151.942848E-5230.142876
TCCTGTA151.942848E-5230.142877
Produced by FastQC (version 0.11.9)