FastQCFastQC Report
Wed 30 Nov 2022
4061-JW13trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW13trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145151
Sequences flagged as poor quality0
Sequence length231
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA6859747.2590612534533No Hit
TAGCTGTAAACGATGGATACTAGGTGTTGCCCGTATCGACCCGGGCAGTG83505.752630019772513No Hit
TAGCCGTAAACAATGGATACTAGGTGTAGCCTGTATCGACCCGGGCTGTG71594.932105187012146No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG59144.0743777169981605No Hit
TAGCCGTAAACAATGGATACTAGGTGTAGCCCGTATCGACCCGGGCTGTG34792.3968143519507272No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC30992.135018015721559No Hit
TAGCCGTAAACGATGGATACTAGGCGTAGCTCGTATCGACCCGAGCTGTG28461.9607167708110864No Hit
TAGCTGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA19041.3117374320535167No Hit
ACGCCGTAAACGTTGGGCACTAGGTGTGGGTCTCAACCAACGAGATCCGC18431.269712230711466No Hit
ACGCTGTAAACGATGAGTGCTCGGTGCTGCGGGTATCGACCCCTGCAGTG12480.8597942831947419No Hit
TAGCCGTAAACAATGGATACTAGATGTTGACCAGATCGACCTGTGCAGTA7910.5449497419928213No Hit
TAGCCGTAAACGATGGACACTAGGTGTTGGCCGTATCGACCCGGTCAGTG7320.5043024161046082No Hit
TAGCCGTAAACAATGGATACTAGATGTTGCACAGATCGACCTGTGCAGTA6540.45056527340493696No Hit
ACGCCTTAAACGATGAATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC6530.44987633567801805No Hit
TAGCCGTAAACGATGAGCACTAGACTGAGGAGACCCCCACATCTTCTCGG5270.36307018208624126No Hit
TAGCCGTAAACGATGGGCACTAGGCAAGGGAGGTACCTATGTCCTTCCTT4510.3107109148404076No Hit
TAGCTGTAAACGATGGGCACTAGCTTGGGGTCTCCCTGTGTGATCCCAGG3830.26386314940992484No Hit
ACGCCTTAAACGATGGATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC3820.263174211683006No Hit
ACGCTGTAAACGATGAACACTAGGTGTCGTGGGTATTGACCCCCGCGGTG3480.2397503289677646No Hit
ACGCCGTAAACGATGATAACTAGCTGTTCGGGCTCTACGAGCTTGAGTGG3120.21494857079868548No Hit
ACGCCGTAAACGATGGGTGCTAGGTGTCGCGGGCTTTGACCCCTGCGGTG3000.20668131807565915No Hit
ACGCCGTAAACGATGTGTGCTCGTTGTCGGGATCTTAGATCCCGGTAACA2950.2032366294410648No Hit
TAGCCGTAAACGGTGCACGTTTGCTGTAAAAGGAATCGACCCCTTTTGTG2920.20116981626030822No Hit
ACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTG2600.1791238089989046No Hit
ACGCTGTAAACGATGAGTGCTAGGCGTCGGCACGCTTGCGTGTCGGTGCC2550.17567912036431027No Hit
ACGCCGTAAACGATGATAACTAGCTGCCAGGGCTCTTAGAGCTTTGGTGG2480.17085655627587823No Hit
ACGCCCTAAACTATGGATACTCGACATACGCGATAAACTGTGTGTGTCTG2440.16810080536820277No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTGAGTATCGACCCTCACAGTG2390.16465611673360844No Hit
ACACTGTAAACGTTGATGACTCGATGTTGGCGATACACAGCCAGCGTCCA2390.16465611673360844No Hit
ACACTGTAAACGATGATTACTCGATGTTGGCGATACACAGCCAGCGTCTT2250.15501098855674436No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA2230.15363311310290662No Hit
TAGCCGTAAACGATGGATACTAAATGTTGCGTGTATTGATCCACGCAGTG2220.15294417537598778No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTTCAGTA2090.14398798492604253No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG1920.13227604356842185No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGGGCAGTA1850.1274534794799898No Hit
ACGCTGTAAACGATGAACACTAGGTGTCGTGGGTATTGACCCCCGCGGTA1610.11091897403393708No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAGCAGATCGACCTGTGCAGTA1600.1102300363070182No Hit
ACACTGTAAACGTTGATAACTCGCTGTTGGCGATACACAGCCAGCGGCCA1580.10885216085318049No Hit
TAGCCGTAAACGATGGGTACTGGGTAGGGGGGAAGCCTATGCTCTTCCCT1580.10885216085318049No Hit
TAGCCCTAAACGATGAGCGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG1520.1047185344916673No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA1480.10196278358399186No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGCCCGTATCGACCCGGGCAGTG1470.10127384585707298No Hit
TAGCCGTAAACAATGGATACTAGGTGTTGCCCGTATCGACCCGGGCAGTG1460.10058490813015411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAA252.6757334E-9224.990723
AAACACT252.6757334E-9224.990728
TTCGGAA252.6757334E-9224.99072225
AACACTG252.6757334E-9224.990729
AACCGAT152.1306505E-5224.99078
CCCTAAA4950.0224.99074
ACACCGT100.0019033746224.99071
ATGGACC152.1306505E-5224.9907225
AACCAAT152.1306505E-5224.99078
ACCGATG202.3812026E-7224.99079
TAAACTA800.0224.99077
CACTGTA850.0224.99072
GGCCCTA900.0224.99072
TAGCCAT100.0019033746224.99071
ATCCGTA152.1306505E-5224.99072
CCGTAAC350.0224.99074
TCGTGAG152.1306505E-5224.9907225
TAACCAA152.1306505E-5224.99077
TGTCTCT100.0019033746224.9907225
GCCCTAA4950.0224.99073
Produced by FastQC (version 0.11.9)