FastQCFastQC Report
Wed 30 Nov 2022
4061-JW13trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW13trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145151
Sequences flagged as poor quality0
Sequence length236
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT6315543.509862143560845No Hit
GTTCGCGCTCCCGAAGGCACTCCCTCCTTTCAAAGGGATTCGCGACATGT112427.745037926021866No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGAAGGTTCGCGACATGT53553.6892615276505154No Hit
GTTCCAGCTCCCGAAGGCACTCTCAGGTTTCCCCGAGATTCTGGACATGT47973.3048342760297897No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAAAAATTCGTATCATGT35252.428505487388995No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC32202.2183794806787414No Hit
CTTGGCGCTCCCTAAGGCACTCTCCTCTTTCAAAGAGATTCGCCAGATGT27831.917313694015198No Hit
GTTCCAGCTCCCGAAGGCACTCTTAGGTTTCCCCAAGATTCTGGACATGT20411.406121900641401No Hit
GTATACGTTCCAAACGGCACTTTCCTCTTTCAAAGAAATTCGTATCATGT18641.284179922976762No Hit
GCAGGATGGTATTGCTACCTCGTTAGGCTTTCACCCTTCTACTACCTGCA9940.6848041005573506No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGCAATTCGTATCATGT7640.526348423366012No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGACATTCGTATCATGT7090.48845684838547443No Hit
GTAGAGCCCCGAAGGACACCCTATCTCTAGAGCTTTTCCCTACATGTCAA7010.48294534657012356No Hit
GTATACGTTCCAAAAGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT6250.4305860793242898No Hit
GTACGACCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA6240.42989714159737097No Hit
ATGGCGGCTCCCGAAGGAGAAGCCGTATTTCTACGACAGTCCACCACAGT6130.42231882660126346No Hit
GTATCCGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT5280.3637591198131601No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT4970.3424020502786753No Hit
CCACTAGCTCCCTTGCGGGCACATCAGACTTTCATCCGACTTCTAATGAC4390.3024436621173812No Hit
GCAAAAGCTCCCCGAAGGGCACCACTCTGTTTCCAAAGTGTTCTTAAGCA4320.29762109802894915No Hit
GTTCGTGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCACGACATGT4310.2969321603020303No Hit
GTACGGCCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA3570.24595076851003436No Hit
ATCCTAGCCAGCCGAACTGAAGGAAATCATCTCTGAAATCCATACTAGGT3310.22803838761014392No Hit
GCAGATGTCCTTGCGGAAGCCGACTTTCGCCGGATGTCATCTACAGTTCA3300.22734944988322509No Hit
CACTAATCCAGCCGAACTGAAGGAATCCATCTCTGGAATCCGCGATTAGG3030.20874813125641573No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT2870.19772512762571393No Hit
CACTGATCCAGCCGAACTGAAGGAAAGTGTCTCCACTATCCGCGATCAGG2760.1901468126296064No Hit
GTATACGGTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2660.18325743536041778No Hit
GCAAGCTGGTATTGCTACCTCGTTAGACTTTCATCCTTCTACTACTTGCA2650.18256849763349892No Hit
GCAGGTTACACCCGAAGGCAATCCGCAGACTTTCATCCGCTTAATCCCTG2610.17981274672582345No Hit
CTGGCAGTGTATTGCTACAAAGTGAGCTTTCACCCACGGTCTCCCAGCAT2590.1784348712719857No Hit
GCAGATTCCGACTCCGAAGAGCTGTCATTCCGCTTTCACGGAACTAATTC2520.1736123071835537No Hit
CTACAGGTTCTCCGAAGAGCACCCCGATCTTTCGACCAGGCTCCTGCATT2510.1729233694566348No Hit
GCAGATTCCGACTCCGAAGAGCTGTCATCCCGCTTTCACGGGACTAATTC2380.16396717900668958No Hit
CACTCTGCTCCCGAAGGAGAAGCCCTATCTCTAGGGTTTTCAGAGGATGT2300.15845567719133868No Hit
CTCCTTGTCCTTGCGGAACGATACATCTCTGTACCCATCAAGGGCATTCT2110.14536586037988025No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2110.14536586037988025No Hit
ATAGGCTCCCCGAGGGGTCGGTCACCTTTCGGATCCCTACCACCTATATG2040.1405432962914482No Hit
GTGCGTGTTCCCCGAAGGGCACTTCCTTCTTTCAAAGAAATTCACGCCAT1880.1295202926607464No Hit
GCAGGATGGTATTGCTACCTCGTCAGGCTTTCACCCTTCTACTACCTGCA1860.12814241720690867No Hit
GTCCGCGTCCATTGCTGGAACCCCACATCTCTGTGAGTAGCACGGCATGT1730.11918622675696343No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC1710.11780835130312571No Hit
CTCCTTGTTCTTGCGAAAAAATCCATCTCTGAATCTGTCAAGGGCATTCT1690.11643047584928798No Hit
GTAAGGCCCCGAAGGACACCCTATCTCTAGAGCTTTTCCCTACATGTCAA1530.10540747221858615No Hit
GTCGAGCCCCGAAGGACACCCTATCTCTAGAGCTTTTCCCGACATGTCAA1480.10196278358399186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAGC3950.0230.071696
CAGGTCT100.001780296230.071674
AGTCCCG400.0230.071676
GGAGCCA100.001780296230.071674
TCCGAAC800.0230.071679
TGGGTCC202.1303276E-7230.071674
TGTATTG400.0230.071678
TTGTACC302.5465852E-11230.071676
TTCCGGT151.9490353E-5230.071676
TTTCCGA100.001780296230.071677
TAGCCAG600.0230.071675
ATTCCGA700.0230.071675
GTGTCCT202.1303276E-7230.071675
TGTTCCG550.0230.071674
GTGTATT400.0230.071677
GCCTATT100.001780296230.071677
GAGCCAG100.001780296230.071675
TCCAAAA550.0230.071679
CTCGAAG550.0230.071679
TGGTATT1550.0230.071677
Produced by FastQC (version 0.11.9)