FastQCFastQC Report
Wed 30 Nov 2022
4061-JW14trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW14trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112771
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA3431130.425375318122565No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA2202719.53250392388114No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA76856.814695267400308No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG54594.840783534774011No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG24072.1344139894121716No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA15141.3425437390818562No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA11941.0587828431068271No Hit
TAGCCCTAAACGATGAGCGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG7290.646442791143113No Hit
TAGCTGTAAACGATGGATACTAGGTGTTGCCCGTATCGACCCGGGCAGTG6890.6109726791462344No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGATGGCGTTCGCGTCGTTCCG6120.542692713552243No Hit
ACACTGTAAACGATGAGCACTCGGTGTTGTGGGTGTCGACCCCTACAGTG4840.42918835516223147No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA4470.3963785015651187No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA4010.3555878727687083No Hit
TAGCCGTAAACAATGGATACTAGATGTTGACCAGATCGACCTGTGCAGTA3770.3343058055705811No Hit
TAGCCGTAAACAATGGATACTAGATGTTGCACAGATCGACCTGTGCAGTA3470.3077032215729221No Hit
TAGCCGTAAACGATGGACACTAGGTGTTGGCCGTATCGACCCGGTCAGTG3350.2970621879738585No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG3200.283760895975029No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTTCTTAGTA2500.2216881999804914No Hit
ACGCCGTAAACGTTGGGCACTAGGTGTGGGTTTCAACCAACGAGATCCGC2200.19508561598283244No Hit
CAGCTGTAAACTATGCTCGCTAGGTGTCAGGGACGGTGCGACCGTTTCTG2050.181784323984003No Hit
TGGCCGTAAACGATGGATACTAGGCGTATCGGGTATCGACCCCTGATGTG1960.17380354878470528No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC1920.1702565375850174No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG1910.16936978478509546No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG1820.16138900958579777No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA1780.1578419983861099No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCTGGGATGGTGCGACCGTTTCAG1720.1525214815865781No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG1660.14720096478704633No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT1520.1347864255881388No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1370.12148513358930932No Hit
ACGCCTTAAACGATGGATACTAAGTGTCGGCGGGTTACCGCCGGTGCCGC1350.11971162798946537No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA1350.11971162798946537No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG1330.11793812238962145No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1320.11705136958969947No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1290.11439111118993357No Hit
ACGCCCTAAACGATGCGAACTGGACGTTGGGCTCAATTGAGAGTTCAGTG1200.10641033599063589No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG1190.10552358319071392No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTTCAGTA1150.10197657199102607No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA1140.1010898191911041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAT100.0019027793224.992028
CCCTAAA8200.0224.992024
TTTGTAA152.1293434E-5224.99202225
ACCGATG100.0019027793224.992029
CACTGTA700.0224.992022
CCTAAAC8200.0224.992025
GGCCCTA500.0224.992022
GCCATAA100.0019027793224.992023
CGTTCAT202.3788016E-7224.992028
TAGCCGG252.6720954E-9224.992021
CCGTCAA100.0019027793224.992024
ATCCGTA100.0019027793224.992022
GGCCATA100.0019027793224.992022
TGGCAGT100.0019027793224.992021
GCCCTAA8050.0224.992023
CCATAAA152.1293434E-5224.992024
CGTAACA152.1293434E-5224.992025
CGTAAAT100.0019027793224.992025
CGTAAAG100.0019027793224.992025
ACGTATA100.0019027793224.99202225
Produced by FastQC (version 0.11.9)