FastQCFastQC Report
Wed 30 Nov 2022
4061-JW14trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW14trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112771
Sequences flagged as poor quality0
Sequence length236
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2743824.330723324258898No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA1351711.98623759654521No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAAAAATTCGTATCATGT55224.896648961169095No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA49164.359276764416384No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA46254.101231699639092No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGAAGGTTCGCGACATGT44943.985067082849314No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA38423.4069042573001926No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA37633.3368507861063574No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC20801.8444458238376888No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT8470.751079621533905No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA5690.5045623431555986No Hit
AGCACACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGTGCTATGTCA5320.4717524895584858No Hit
GTTCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGACAT5140.4557909391598904No Hit
GCACGAGTCCCGAAGGAAGACCTCATCTCTGAGGCTGTCTCGTACAGTTC3930.3484938503693325No Hit
ATGATGGTCCCCGAAGGGAAAGCCCACTTTCATGGACAGTCCACCACAGT3830.33962632237011287No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC3560.3156839967722198No Hit
GCACGAGTCCCGAAGGAAGACCTCACTTCTGAGGCTGTCTCGTACAGTTC3250.2881946599746389No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGCAATTCGTATCATGT3130.2775536263755753No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC3100.2748933679758094No Hit
GTTCCAGCTCCCGAAGGCACTCTCAGGTTTCCCCGAGATTCTGGACATGT2840.2518377951778383No Hit
GTTCGTGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCACGACATGT2760.24474377277846254No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGACATTCGTATCATGT2680.23764975037908684No Hit
CAGCAGGGTTCCGGACATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT2420.21459417758111574No Hit
GTTCCGATTCCCTTTCGGGCACCCCCAGCTCTCACCAGGGTTCCGGACAT2310.2048398967819741No Hit
GTACGGCCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA2280.2021796383822082No Hit
GTATCCGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2210.19597236878275445No Hit
CAGCGATTCTGGTAAAGTCTTTAGCTTGACCTACATATTGCTGTCGCCCC1850.16404926798556366No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC1830.16227576238571972No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC1740.15429498718642204No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA1690.14986122318681222No Hit
CTCCTGGCTCCTTGCGGCACGTCCATGCATCTCTGCTAGACTTCCAGGGA1570.13922018958774862No Hit
GTTCCAGCTCCCGAAGGCACTCTTAGGTTTCCCCAAGATTCTGGACATGT1500.13301291998829487No Hit
GTCCCGATTCCCTTTCGGGCACCCCCGCCTCTCAGCAGGGTTCCGGGCAT1390.12325863918915324No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC1350.11971162798946537No Hit
CAGCGATTCAGGTAAGACCTTCAGCCTGACCTACATATTGCTGTCGCCCC1330.11793812238962145No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCGGGATTCCTGGCAT1280.11350435839001163No Hit
CAGCAGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT1250.1108440999902457No Hit
GTTCGCGCTCCCGAAGGCACTCCTCCCTTTCAAGAGGATTCGCGACATGT1220.1081838415904798No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1160.10286332479094802No Hit
GCAGGATGGTATTGCTACCTCGTCAGGCTTTCACCCTTCTACTACCTGCA1150.10197657199102607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT100.001779385230.08832
CAGGTTA100.001779385230.08832
GGCAGTG100.001779385230.08833
GGCAGTC100.001779385230.08834
AAATCAG100.001779385230.08835
ACGCCTC100.001779385230.08832
CTGTATT100.001779385230.08839
ATGATGG400.0230.08831
ACGCCAG100.001779385230.08835
GTCTGGT202.1274536E-7230.08837
GATCGCG100.001779385230.08831
GATCGAG252.3374014E-9230.08831
ATGGCAG100.001779385230.08831
CGGCATT100.001779385230.08833
GGTCACA100.001779385230.08834
TTCCGAC100.001779385230.08836
GCCATTG100.001779385230.08837
GGATACG100.001779385230.08831
AGACGTT100.001779385230.08833
TGGCAGT100.001779385230.08832
Produced by FastQC (version 0.11.9)