FastQCFastQC Report
Wed 30 Nov 2022
4061-JW15trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW15trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129727
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA6201547.80423504744579No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA88356.8104558033408615No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA77325.960208746059031No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA47593.668473024119882No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG13481.0391051978385377No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG10910.8409968626423181No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG6380.4918020150007323No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG5990.4617388824223177No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG5380.4147170596714639No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT5280.40700856413853714No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG5230.40315431637207366No Hit
TAGCTGTAAACGATGTGAACTTGGCGTTGGTGGCTTAAACTCCATCAGTG5050.3892790244128053No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT4890.3769454315601224No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG4750.3661535378140248No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG4380.33763210434219554No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC3980.3067981222104882No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG3800.2929228302512199No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA3560.2744224409721955No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA2930.22585891911475636No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2930.22585891911475636No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2760.21275447670878075No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG2750.21198362715548805No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT2750.21198362715548805No Hit
TAGCAGTAAACGATGTAGACTTGGTGTCGGAGGTGTCAAAACCTTCGGTA2580.1988791847495124No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2370.18269134413036608No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGATCAGTGTCGCA2350.1811496450237807No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG2330.17960794591719534No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA2240.1726702999375612No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2190.16881605217109777No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG2120.16342010529804898No Hit
TAGCAGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2070.15956585753158556No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC1970.15185736199865874No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1920.1480031142321953No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1840.14183631780585385No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGTTCAGTGTCGCA1790.1379820700393904No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG1760.13566952137951235No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1760.13566952137951235No Hit
CAGCTGTAAACGATGCTCGCTAGGTGTCAGGTGCGGTGCGACCGCATCTG1590.12256507897353675No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG1590.12256507897353675No Hit
TAGCCGTAAACGATGTAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1500.11562743299390259No Hit
ACGCCGTAAACTATGGGTACTAGGTGTAGGGGGTATCGACTCCCCCTGTG1480.1140857338873172No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG1390.10714808790768306No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1370.1056063888010977No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG1330.10252299058792695No Hit
AGGGTGTAAACGCTGCTCGCTTGATGTTAGTCGGGCTCCGAGCCCAACTA1310.10098129148134159No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGCCATATTGTTCAGTGTCGCA1310.10098129148134159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG303.092282E-11225.000029
AACAATC152.1297777E-5225.000029
ACCGATG303.092282E-11225.000029
TAAACCA152.1297777E-5225.000027
CAGTAAA2400.0225.000024
GTAAATG152.1297777E-5225.000026
TTGGACC100.0019029061225.0225
AACCAAG100.0019029061225.09
TAAACAA400.0225.07
GGCCCTA400.0225.02
TGGCCGT850.0225.01
CTGAATC1050.0225.0225
AAACGCT1450.0225.08
AAACCAA100.0019029061225.08
AACGATA1050.0225.09
AAACAAT450.0225.08
CGTACAC100.0019029061225.05
CATAAAC100.0019029061225.05
CGTAACC252.6739144E-9225.05
GACGATG100.0019029061225.09
Produced by FastQC (version 0.11.9)