FastQCFastQC Report
Wed 30 Nov 2022
4061-JW15trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW15trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129727
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA4445234.26580434296638No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA2575219.850917696393193No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA74705.758246163096349No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA55654.2897777640737855No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC11410.8795393403069522No Hit
CAGCGAATTCGGTAAAGTCTTTAGCTTGACCTTCATATTGCTGTCTGACC10260.7908916416782936No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC7770.5989501029084154No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA6900.5318861917719518No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC4480.3453405998751224No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3870.29831877712426863No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA3720.2867560338248784No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA3630.27981838784524427No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC3390.26131799856621984No Hit
TCAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCTGTATG3290.253609503033293No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA3240.24975525526682957No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT3230.24898440571353692No Hit
TACAGTTCCCCGAAGGACCTCTTCTTTCAAAGAGTTTAACTGCAATATCA3090.23819251196743932No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2800.21583787492195147No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT2620.20196258296268318No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC2550.19656663608963437No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT2520.19425408742975633No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT2460.18962899011000023No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2400.18500389279024412No Hit
ACAGGTTCCTCCAAAGGAGGATATCTATCCCTTTCAGGTAGATATGCCTG2260.17421199904414655No Hit
GAAGGCTCCCTTGCGGGTCGGTCACCTTTCGGATTCCTACCACCAACATG1980.1526282115519514No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1970.15185736199865874No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1910.14723226467890263No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT1870.14414886646573188No Hit
GAGGGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACCCACATG1860.1433780169124392No Hit
ACTGGAGTCCCCGAAGGGAAGGCCTCATCTCTGAGGCTGCCCCCAGCAGT1840.14183631780585385No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1780.13721122048609774No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA1750.13489867182621967No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG1660.12796102584658553No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT1650.12719017629329285No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1640.12641932674000014No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA1610.1241067780801221No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC1600.12333592852682942No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1600.12333592852682942No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAATGAGTTTAGCTGCAATATCA1440.11100233567414648No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1410.10868978701426843No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1400.10791893746097574No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC1360.104835539247805No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1330.10252299058792695No Hit
CCACGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACGTGCATG1320.10175214103463429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCA252.3446773E-9229.997314
CTGCTAG302.5465852E-11229.99731230
GTACCCG151.9510748E-5229.997318
CCCTCGA450.0229.997317
AATCAGG500.0229.997316
TTGGACC151.9510748E-5229.99731230
CAAGTAG650.0229.997319
TCCTGAA151.9510748E-5229.997318
CATTGCT151.9510748E-5229.997319
ATCAGGC450.0229.997317
GGTCCAG302.5465852E-11229.997315
AGGACAG151.9510748E-5229.997311
GCCATTG151.9510748E-5229.997317
GTGGACA2450.0229.99731230
ACTAGCT151.9510748E-5229.997311
CCGGTTC252.3446773E-9229.997314
CCGCGCT151.9510748E-5229.997313
GGACAGC151.9510748E-5229.997312
GCTCGTC900.0229.997313
AGCTAAT1000.0229.997312
Produced by FastQC (version 0.11.9)