FastQCFastQC Report
Wed 30 Nov 2022
4061-JW16trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW16trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123572
Sequences flagged as poor quality0
Sequence length231
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA5877147.56012688958664No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA33262.6915482471757355No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA30852.496520247305215No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG28232.284498106367138No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG18691.5124785550124622No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA15551.2583756838118667No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG12511.0123652607386786No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG11400.9225390865244553No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG7750.6271647298740815No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG7240.5858932444243032No Hit
TGGCTGTAAACGATGGATACTAGATCTTGCCTGAAGGGCTTGGTCGTAGC6590.5332923315961545No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG6360.5146797009031172No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG6100.4936393357718577No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG5400.4369921988800052No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT5210.4216165474379309No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG4850.3924837341792639No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT4730.3827727964263749No Hit
ACGCCGTAAACGATGAGAACTAGGTGTTGCGGGTATTGACCCCTGCAGTG4240.34311980060207814No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG4130.3342181076619299No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG3990.3228886802835594No Hit
GTGCCGTAAACGATGTGGACTAGGTGTCGGCGACGCGAGTCGGCGGTGCC3950.3196517010325964No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3710.3002298255268184No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT3440.2783802155828181No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG3320.2686692778299291No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC3260.2638138089534846No Hit
ACGCCGTAAACGATGAATGCCAGCCGTTAGTGGGTTTACTCACTAGTGGC3260.2638138089534846No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3200.2589583400770401No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG2990.24196419900948435No Hit
ACGCCGTAAACGATGATTACTCGTTGTTGGCGATATATTGTCAGCGACTG2660.21525912018903962No Hit
ATGCTGTAAACGATGTGAACTAGGTGTTGCGCCGTCAAGGGGCAGTGCCG2650.21444987537629887No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG2580.20878516168711359No Hit
ACGCTGTAAACGTTGTGTACTAGGTGTTAGTGACTTGTTCACTAGTGCCG2430.19664648949600233No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2410.19502799987052083No Hit
GTGCCGTAAACGATGCAGACCAGGTGTGGCGGGCGCAAGCCTGCTGTGCC2390.19340951024503933No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA2350.19017253099407633No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2290.18531706211763183No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG2290.18531706211763183No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG2250.18208008286666882No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2180.17641536917748357No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG2090.16913216586281682No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG2050.16589518661185382No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA1980.16023047292266857No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGGGGTATTGATCCCTTCGGTG1940.15699349367170556No Hit
ACGCCGTAAACGATGAGTACTAGGTGTCGGGGGTTACCCCCCTCGGTGCC1940.15699349367170556No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGACTTGTTCACTAGTGCCG1910.1545657592334833No Hit
ACGCTGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA1870.1513287799825203No Hit
ACGCCGTAAACTATCCCTGTTAGGTGTTTGGTGGTTCACTGAGATTTTTT1850.1497102903570388No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG1740.14080859741689056No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG1720.13919010779140906No Hit
ACGCCCTAAACGATGGGCACTAGGTGTGGGAGGTATCGACCCCTTCCGTG1710.1383808629786683No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG1690.1367623733531868No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG1670.1351438837277053No Hit
ACGCCGTAAACGATGGGCACTAGGTGTCGCGGGTATTGACCCCTGCGGTG1650.1335253941022238No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA1640.13271614928948305No Hit
ACGCTGTAAACGATGTTCACTAGGTGTTGGGGGTATTGACCCTCTCAGTG1640.13271614928948305No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCATCGAG1640.13271614928948305No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG1590.1286699252257793No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA1580.12786068041303855No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG1580.12786068041303855No Hit
ACGCTGTAAACTTTGGGCACTAGGTATTGGAGGTCTCAACCCCTTCAGTG1560.12624219078755705No Hit
ACGCCGTAAACGATGAATACTAGGTGTCGGGGGTCGAACCTCGGTGCCGC1530.1238144563493348No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG1520.12300521153659405No Hit
ATGCCGTAAACGATGTGCACTAGGTGTAGGGGTGTCACAGCCGCTGTACC1520.12300521153659405No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA1470.1189589874728903No Hit
ACGCCGTAAACGATGAATACTAGGTGTTGGGGGTCGAACCTCGGTGCCGC1430.11572200822192728No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGTGGGAGTTGACCCCTGCCGTG1410.1141035185964458No Hit
TAGCCGTAAACGATGGATACTAGGTGTTAGAGGTATCGACCCCTCTAGTG1400.11329427378370505No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC1380.11167578415822355No Hit
ACGCTGTAAACGATGAATACTAGGTGTTGGGTTTTAAACTCAGTGCCGCA1370.1108665393454828No Hit
ACGCCGTAAACGGTGAGCACTAGGTGTAGCGGGTATTGACCCCTGCTGTG1330.10762956009451981No Hit
CAGCTGTAAACGATGCAAACTAGGTGTTTGAACGGCCACGTGCCGTTCTA1300.10520182565629753No Hit
ATGCCGTAAACGATGCCGACTAGGTGTGGGGCGGGTCAAACCGTTCTGTG1280.10358333603081604No Hit
ACGCGGTAAACGATGTGCACTAGATGTCGGAGGTATTGATCCCTTCGGTG1250.10115560159259379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG1900.0225.000029
GCCCTAA3950.0225.000023
AGCGTTC100.0019027975225.06
TGCCCTA100.0019027975225.02
CACGGTA100.0019027975225.02
CAACGAT100.0019027975225.08
ACACCGT152.1295344E-5225.01
AACCATG100.0019027975225.09
ACCGATG152.1295344E-5225.09
TAAACAT100.0019027975225.07
CACTGTA1100.0225.02
TAGCCTT100.0019027975225.01
TAGCCAT100.0019027975225.01
TGGCCCT650.0225.01
CGGGGTA100.0019027975225.0225
CAGATTT152.1295344E-5225.0225
AACGATC202.3792927E-7225.09
ACCGTAA202.3792927E-7225.03
ACGTAAA100.0019027975225.04
AACTATC252.6720954E-9225.09
Produced by FastQC (version 0.11.9)