FastQCFastQC Report
Wed 30 Nov 2022
4061-JW16trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW16trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123572
Sequences flagged as poor quality0
Sequence length236
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA4117233.318227430162175No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA123509.994173437348266No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA61094.943676561033244No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA35762.893859450360923No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC22001.7803385880296507No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC21871.7698184054640211No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC10930.8845045803256402No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC10730.868319684070825No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT6710.5430032693490435No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT5820.47098048101511675No Hit
GCAGGTTCCTTCAAAGAAGGATCACTAGCCCTTTCAGGTAGCTATGCCTA5440.4402291781309682No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG5250.4248535266888939No Hit
TACGACTCGCCGAAGCACCTCATCTTTCAATGAGTTTAGTCGCAATATCA5170.4183795681869679No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT5170.4183795681869679No Hit
TACGACTCGCCGAAGCACCTCATCTTTCGATGAGTTTAGTCGCAATATCA4340.35121224872948564No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT4270.3455475350403004No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA3790.3067037840287444No Hit
ACGAAGGCGTATTGCTACGCTGCGATATCGCTACCGCATTCCTCCGTATG3660.2961836014631146No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3450.27918946039555886No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC3350.27109701226815136No Hit
GCTCCAGGCTCCGAAGAGAAGGTCACATCTCTGCGACCGGTCCTGGACAT3200.2589583400770401No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC3150.25491211601333635No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC3040.2460104230731881No Hit
CACCGATCCAGCCGAACTGACCCTCAAGTTTCCAGGAGGTACGATCGGGA2840.22982552681837307No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2800.2265885475674101No Hit
CACCCTGTCCCGAAGGACTTCGCCCATCTCTGGGTTATGCAGGGGATGTC2670.21606836500178034No Hit
CTTACGGCTCCCCGAAAGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT2340.18936328618133558No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2250.18208008286666882No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT2240.18127083805392807No Hit
ACAGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCTGTATGTC2210.17884310361570582No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2160.17479687955200207No Hit
CAGCGATTCAGGCAAGGTCTTCAGCCTGGCCTACATTTTGCTGTCGTCCC2140.17317838992652057No Hit
ATTAGCTCCTGTATTGCTACAGGTCGGTCCCCTTTCAGTTTCCTACCACT2050.16589518661185382No Hit
ATACCTGTCCGAAGAAGGGTGTATCTCTACACCTGTCAGGTACAGTTCAA2000.16184896254815007No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT1940.15699349367170556No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA1890.1529472696080018No Hit
ACAAGTGCCATTGCTGGAGGGGTACTTTCATACCCTGTCACTTGCCTTTC1860.15051953516977956No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC1810.1464733111060758No Hit
ATAGCACCCTCGAAGGCTGAATCCATTTCTGGAAACGTGCAGCTATAGTT1810.1464733111060758No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1710.1383808629786683No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA1700.13757161816592756No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1660.13433463891496455No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA1640.13271614928948305No Hit
TAACCTGCCAATTGCTTGGAAATACCCTTTCAGGTACGGTCACGCTACAT1610.1302884148512608No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT1580.12786068041303855No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC1510.1221959667238533No Hit
CTCCAGGTTCTCTCTAATGAAAGCACTCTCCTGTTTCCAGGAGATTCCCG1440.11653125303466806No Hit
GCTGGGGTCCCCGAAGGGAAGACCGTATCTCTACGGCTGACTCCAGCAGT1380.11167578415822355No Hit
CAGCTAGTCAAGCAGAGTCTTCAGCCCGGCTATCATTCAGCTGTCTTATC1320.10682031528177906No Hit
CAGCTTGTCTGGTAAGGTCGTCAGCCTGACCTTCATTCTGCTGTCGCCCC1310.10601107046903828No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG1300.10520182565629753No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATACAGCTGTCTTATC1290.10439258084355679No Hit
CACGGCTCCCGAAGGTCGGTCCGGTTTCCCTTCCCTACCACCGTGATGTC1290.10439258084355679No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCACTTCCCTACCACTTGCATG1270.10277409121807529No Hit
CACCGATCCAGCCGAACTGACCCCGCTGTTTCCAGCAGGTTTAATCGGGA1260.10196484640533454No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGTGAGTTTAGCTGCAATATCA1250.10115560159259379No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA1240.10034635677985304No Hit
CTCCTGGCTCTCTTGCGAGCACCCCCCCGTTTCCGGGAGGTTCCAGGGAT1240.10034635677985304No Hit
CAGCTTGTCTGGTAAAGTCTTCAGCTTGACCTTCACTCTGCTGTCGCCCC1240.10034635677985304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG100.0017817036229.99718230
CATGGTG100.0017817036229.997181
CATGGGC100.0017817036229.997181
ATGGGCC100.0017817036229.997182
CTCACTG151.9508558E-5229.997181
ATCTCGA202.1324922E-7229.997187
TTGGACT151.9508558E-5229.99718230
TGCCATT151.9508558E-5229.997186
ATAGGCT400.0229.997181
AGATAAC100.0017817036229.997186
CGAAAAA151.9508558E-5229.997181
CAGCCGA1550.0229.997189
GTCCTTG151.9508558E-5229.997187
GTGGGTG100.0017817036229.99718230
GACGTCC650.0229.997184
GGCGTAT302.5465852E-11229.997186
CAGCAAG202.1324922E-7229.997182
TCTTACG202.1324922E-7229.997189
GGTCCAG151.9508558E-5229.997185
GCAAAGC100.0017817036229.997181
Produced by FastQC (version 0.11.9)