FastQCFastQC Report
Wed 30 Nov 2022
4061-JW17trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW17trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191165
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA10964257.354641278476706No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA49912.6108335730913086No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA44992.3534642847801637No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG30221.5808333115371536No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA27211.423377710354929No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT15290.7998326053409358No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG14420.7543221824078675No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG13260.6936416184971098No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT9920.5189234430988936No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG9770.5110768184552612No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG9620.5032301938116287No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG8870.46399707059346634No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG5700.29817173645803363No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT5680.29712551983888263No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT5470.2861402453377972No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG5430.2840478120994952No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA4860.2542306384536918No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA4710.24638401381005937No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG4700.2458609055004839No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA4580.23958360578557789No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG4230.22127481495043547No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG3990.20872021552062353No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG3930.20558156566317054No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG3850.20139669918656658No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG3810.19930426594826461No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA3580.18727277482802815No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA3470.18151858342269767No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC3460.18099547511312217No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCAACGGTGCGACCGTTGTCG3400.17785682525566918No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC3300.1726257421599142No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG3300.1726257421599142No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG3160.16530222582585724No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG3150.16477911751628174No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3110.16268668427797975No Hit
CAGCTGTAAACGATGCAGGCTAGGTGTTTGGACGGCCACGTGCCGTTCTA3070.16059425103967778No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG3030.15850181780137576No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2960.1548400596343473No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT2960.1548400596343473No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2910.1522245180864698No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG2820.14751654330029035No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG2720.14228546020453536No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG2700.14123924358538437No Hit
TAGCCGTAAACGATGTGAACTGGGTGTCGGTGGGGTAAAAGCCATCGGCG2630.13757748541835588No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2530.1323464023226009No Hit
TAGCAGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT2460.12868464415557243No Hit
ACGCCGTAAACGATGAATGCCAGCCGTTAGTGGGTTTACTCACTAGTGGC2320.12136112782151545No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTTTCGCA2210.11560693641618498No Hit
TGGCCGTAAACGATGTGGACTTGGTGTTGGAATGGCCTCGAGCTGCTCCA2180.11403761148745847No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGTGGGTTTAAATCCATCGGTG2060.10776031177255249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT152.1313519E-5225.000021
CACGGTA350.0225.000022
CAACGAT303.092282E-11225.000028
GCTATAA152.1313519E-5225.000023
TGGCCGT600.0225.000021
AAACGGT1400.0225.000028
ACGGAGC152.1313519E-5225.00002225
CCATAAA303.092282E-11225.000024
CGTACAC152.1313519E-5225.000025
CATAAAC303.092282E-11225.000025
CGTAAAT152.1313519E-5225.000025
ACGTAAA152.1313519E-5225.000024
ACCATAA100.0019036094225.03
CCCTAAA4350.0225.04
TAAACAA202.382676E-7225.07
ACCCTAA100.0019036094225.03
GCCATAA202.382676E-7225.03
AAACGTT2450.0225.08
AGACGAT100.0019036094225.08
AGGCCGT202.382676E-7225.01
Produced by FastQC (version 0.11.9)