FastQCFastQC Report
Wed 30 Nov 2022
4061-JW17trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW17trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191165
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA10102552.847016974864644No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA111965.856720634007271No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA73793.8600162163575966No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC25541.3360186226558208No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC16830.8803912850155625No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA12680.663301336541731No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC12540.655977820207674No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA10440.5461250751968195No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA7940.41534799780294507No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA6170.32275782700808203No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT5840.3054952527920906No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC5650.29555619491015617No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT5070.2652159129547773No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC4910.25684618000156934No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC4750.24847644704836133No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT4320.22598278973661495No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG4220.22075170664086No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT4210.22022859833128447No Hit
CTTACGGCTCCCCGAAAGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT3190.16687155075458374No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATCCAGCTGTCTTATC2950.1543169513247718No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2940.15379384301519627No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT2880.1506551931577433No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2770.14490100175241283No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC2720.14228546020453536No Hit
GCTCCAGGCTCCGAAGAGAAGGTCACATCTCTGCGACCGGTCCTGGACAT2490.1302539690842989No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG2390.1250228859885439No Hit
GCAGGGCCCTCTCGGACACGACGTTTCCGCCGCTTTACCCTGCATGTCAA2340.12240734444066645No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG2300.12031491120236444No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2270.11874558627363796No Hit
TACAGCGTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA2250.11769936965448696No Hit
TACAGCTTCCCGAAGGACATCATCTTTCAATGAGTTTAGCTGCAATATCA2110.11037585332043No Hit
CAGCTTGTCAAGCAAGGTCGTCAACCTGGCCATCATTCTGCTGTCGCCCC2040.10671409515340151No Hit
CACCGATCCAGCCGAGCTGACCCTCCTGTTTCCAGGAAGTACGATCGGGA2010.10514477022467501No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC2000.10462166191509953No Hit
GCATGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACATGCATG1990.10409855360552403No Hit
CCACGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACGTGCATG1980.10357544529594853No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT1960.10252922867679753No Hit
GGCCGCGCCTATTGCTAGGGGCTCCTGTTTCCAGGAGTTACACGGTCATG1940.10148301205764655No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG1930.10095990374807103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTGT400.0230.000022
GAGGACG100.0017823982230.00002230
AGTCAGG100.0017823982230.000026
GATCGAG100.0017823982230.000021
GGGCTAG100.0017823982230.00002230
CTATCTG100.0017823982230.000021
TGGACGC100.0017823982230.000022
CATTGTC202.1354026E-7230.000024
TTGTCTC100.0017823982230.000026
GGTCCGA202.1354026E-7230.000026
ATAGCAC202.1354026E-7230.000021
ATTGTCT100.0017823982230.000025
ATTCGGT100.0017823982230.000027
AATGGCT202.1354026E-7230.000021
GTGGCGT100.0017823982230.00002230
GTGGCCA400.0230.00002230
TACGCGC100.0017823982230.000022
GTATGCT100.0017823982230.000021
CTGTTGA100.0017823982230.000028
AACTGTT400.0230.000023
Produced by FastQC (version 0.11.9)