FastQCFastQC Report
Wed 30 Nov 2022
4061-JW18trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW18trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189420
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA9840351.94963573012353No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA80194.2334494773519165No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA71833.7921022067363532No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATTTTGATCAGTGTCGCA52392.76581142434801No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA31181.6460774997360361No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG23311.23059866962306No Hit
ACGCCGTAAACGATGGGTGCTTGGTGTCGGGGGTATCGACCCCTCCGGTG14000.7390983000739099No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCGACTCCCTCCGT13910.734346953859149No Hit
TAGCGGTAAACGATGGAAACTAGGTGTTGCCTGTATCGACCCGGGCAGTG10070.5316228486960195No Hit
ACGCTGTAAACACTCCCTGTTAGATATTTGGTAGTTCATTGAAATTTTTT8880.46879949318973707No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG8040.4244535951853025No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA7700.40650406504065045No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTCATCAACTCCCTCCGT7160.3779959877520853No Hit
ATGCCGTAAACGATGCTGACTAGGTGTGGGAGAGGTGAAACTCTACTGTG4840.25551684088269455No Hit
ACGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTG4800.25340513145391197No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGGCATATTGATCAGTGTCGCA4770.25182134938232503No Hit
TGGCAGTAAACGATGTGAACTTGGCGTCGGCGGGATAAAACCTGTCGGTG4650.2454862210959772No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG4350.22964840038010773No Hit
ACGCCGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG4320.22806461830852076No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGGGTCGCA4300.22700876359412944No Hit
ACGCCGTAAACGATGAACACTAGTTGTGTGCGATACACAGTACGCGACTG4180.22067363530778164No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG4130.2180339985218034No Hit
ACACTGTAAACGATGTACACTAGGTGCTGGGGCGGATGCTTCAGTGCCGT4110.21697814380741212No Hit
TAGCTGTAAACGATGTAGACTTGGCGTCGGTGGTGTAAAAGCCATCGGTG3850.20325203252032523No Hit
TAGCCGTAAACGATGGGTACTAGGTGTAGGGGGTATCGACCCCCCCTGTG3710.19586104951958613No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG3710.19586104951958613No Hit
ACGCTGTAAACGATGGGTACTGGGTTTCGGGGGTCTCAACCCTCTCGGAG3690.19480519480519481No Hit
TAGCTGTAAACGATGCAGACTTGGCGTCGGTGGGGTAAAAGCCATCGGTG3540.18688628444726005No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGGCGCA3480.18371872030408615No Hit
ACGCCCTAAACGATGGGCACTAGGCGTCGCGGGTGTCGACCCCTGCGGTG3390.1789673740893253No Hit
CAGCTGTAAACGATGCAGACTAGGTGTTTGGACGGCCACGTGCCGTTCTA3240.17104846373139057No Hit
ATGCCGTAAACGATGCAGACTAGGTATCGCGGGGGTAAAACCCGGCGGCG3190.16840882694541232No Hit
ACGCTGTAAACAATCCCTGCTAGATGTTGGTTTGACTCTTGTTTTTACTT3180.16788089958821667No Hit
ACGCCGTAAACGATGGGCACTTGGTATCGCGGGTATTGACCCCTGCGGTG3160.16682504487382535No Hit
TAGCCGTAAACGATGGGCACTAGGTGTGGGGGGTATAACTCCCTCCGTGC3160.16682504487382535No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG3130.16524126280223841No Hit
ACGCCGTAAACGATGGGCACTAGGTGTGGGAGGTGTCGACTCCTTCCGTG3120.16471333544504277No Hit
TAGCCGTAAACGATGTGAACTGGGTGTCGGTGGGGTAAAAGCCATCGGCG3010.1589061345158906No Hit
ACGCCCTAAACGGTGGGTACTAGGTGTAGGGCTCGCAAGGGTTCTGTGCC2990.15785027980149932No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTTGAGGGCGCCCA2970.156794425087108No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATGATTTTGCTTGGTCGAAG2930.15468271565832542No Hit
TAGCCCTAAACGATGAATACTAGGTGTTGGAGGATTCACTCCTTCAGCGT2880.15204307887234716No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA2850.15045929680076023No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2820.14887551472917326No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG2810.14834758737197762No Hit
TAGCTGTAAACGATGTAGACTTGGCGTAGGTTGGGTAAAACCGATCTGTG2780.14676380530039065No Hit
ACACGGTAAACGATGAATGCTAGGTGTAGGGGGTATCGACCCCTTCTGTG2700.14254038644282546No Hit
ACGCCGTAAACGATGAATGCCAGCCGTTAGTGGGTTTACTCACTAGTGGC2630.13884489494245592No Hit
ACGCTGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA2390.1261746383697603No Hit
TAGCCGTAAACGATGTAGACTTGGTGTTGGAGGGGTAAAAGCCTTCAGTG2370.12511878365536902No Hit
ACGCAGTAAACGATGTGCACTAGGTGTTGGGGGTAATAAAACCTTCAGTG2190.11561609122584732No Hit
TAGCTGTAAACGATGGATACTAGATCTTGCATCAATTTGCTTGGTCGTAG2100.11086474501108648No Hit
TAGCCGTAAACGATGTAGACTTGGTGTCGGCGGGGTAAAAGCCGTCGGTG2080.10980889029669517No Hit
TAGCTGTAAACGATGTGAACTTGGCGTCGGCGGGTTCAAAGCCGTCGGTG1950.10294583465315173No Hit
ACACTGTAAACGATGTGCACTAGGTGCTGGGGCGGATGCTTCAGTGCCGG1930.10188997993876042No Hit
ACGCCGTAAACTATCCCTGTTAGGTGTTTGGTGGTTCACTGAGATTTTTT1920.10136205258156478No Hit
ACGCCGTAAACGATGGGCACTAGGTGTCGCGGGTATTGACCCCTGCGGTG1900.10030619786717347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAATC152.1313213E-5225.00002225
AACAATG303.092282E-11225.000029
CTGTCAA152.1313213E-5225.000024
ACCGATG152.1313213E-5225.000029
AACTATC303.092282E-11225.000029
CAACGAT202.3826215E-7225.08
AGCCCTA700.0225.02
TAAACAA800.0225.07
TAGCCCT700.0225.01
AGACGAT100.0019035959225.08
AGGCCGT202.3826215E-7225.01
CGTAGAC100.0019035959225.05
TACGACG100.0019035959225.0225
TTGACTA1600.0225.0225
TCGCTGT100.0019035959225.01
AACGATC350.0225.09
ACTGTCA100.0019035959225.03
TCATAGC100.0019035959225.0225
ACGTAAA252.6775524E-9225.04
GACGATG100.0019035959225.09
Produced by FastQC (version 0.11.9)