FastQCFastQC Report
Wed 30 Nov 2022
4061-JW18trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW18trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189420
Sequences flagged as poor quality0
Sequence length236
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA8759146.24168514412417No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA170168.983211910041177No Hit
TACAACTCGCCGAAGCACCCTCCCTTTCAGGAGGTTTAGTTGCAATATCA71703.7852391510928096No Hit
GACGACTCCCCGAAGGACCTCATCTTTCGATGAGTTTAGTCGCCATATCA56612.988596769084574No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC19521.0305142012459085No Hit
GCAGGGCCCTTTCGGACGCGACGTTTCCGCCGTTTTTCCCTGCATGTCAA9410.4967796431211065No Hit
GTAGCACCTTCGAAAGCTGAATCCATCTCTGGAAACGTGCAGCTACAGTT7850.41442297539858514No Hit
GTTCAGGCTCCCTTTCGGGCACAATCTCATCTCTGAAATCTTCCTGACAT7680.4054482103262591No Hit
GTCCAGGTTCCCTTTCGGGCACAATCACATCTCTGCGATCTTCCTGGCAT7410.39119417168197657No Hit
CTTCTGGTTCCCTAAGGCACTCTCTCGTTTCCAAGAGATTCCAGAGATGT7100.3748284236089114No Hit
GTCCAAGTTCCCTTTCGGGCACAATCACATCTCTGCGATCTTCCTGGCAT6610.3489599831063246No Hit
GTGACAGCTCCTTGCGGAGAGGAGGACTTTCATCCCCTGTCTGCCCCTCT6120.32309154260373774No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC5850.3088375039594552No Hit
GACGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCGCCATGTC5710.3014465209587161No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC5680.2998627388871291No Hit
GCAGGGCCCTTGCGGACACGACGTTTCCGCCGCTTTTCCCTGCATGTCAA4610.24337451166719462No Hit
CAGTCCGCCCGAAGGGGTTCCAATTTCTCGGAATTTCGTCCTGCATTTAA4480.23651145602365115No Hit
CTTCCTGCTCCGAAGAGCTTCCCCCATTACGGGTAATTCAGGAGATGTCA4340.22912047302291205No Hit
CTTACGGCTCCCCGAAGGGCACCCCCTGCTTTCACAGAGGTTCCGTAGAT3650.1926934853764122No Hit
GTGGACTAACCCTGCTTTCACAGGGCTGACCCCGCCCGAAGGCGGAGCTC3620.19110970330482527No Hit
CCACGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACGTGCATG3530.1863583570900644No Hit
GTAGCACCCTCGAAGGCTGTTCCTCTTTCAAGAAACATGCAGCTACAGTT3300.17421602787456447No Hit
CAGCTCGTCTGGCAAGACCTTTAATCTGGCCTTCACACTGCTGTCGCTCC3280.17316017316017315No Hit
GCTCCAGGCTCCGAAGAGAAGGTCACATCTCTGCGACCGGTCCTGGACAT3200.16893675430260796No Hit
ATAGGCTCCCCGAAGGGTCGGTCACATCTCTGCTCCCTACCACCTATATG3110.16418540808784712No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT3020.15943406187308626No Hit
ATGACGACCCCGAAGGGAGGGAGTGTTTCCACTCCTGTCCGTCACGGTTC2960.15626649772991236No Hit
AGGACAGCTCCTTGCGGAGAGGAGGGCTTTCACCCCATGTCTGCCCCTCT2880.15204307887234716No Hit
ACAGGCTCCCCGAAGGGTCGGTCACCTCTCGGATCCCTACTACCTGTATG2830.1494034420863689No Hit
GAAGGCTCTCTTACGAGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2730.14412416851441243No Hit
GAAACTGTTTTCATATTGCTATGAAAAAGGATCTAGTTTCTTAGATCTTT2720.14359624115721675No Hit
ACAGCGTCGTATTGCTACGCTAGATCATCTCTGACCTATCACACTGTATG2700.14254038644282546No Hit
ACTGGCTCCCGAAGGTCGGTCCCCTTTCGGTTCCCTACCACCAGTATGTC2670.14095660437123853No Hit
GAAGGCTCCCTTACGGGTCGCTCCGCTTTCGCTTCGCTACCACCCCCATG2640.13937282229965156No Hit
CGGAAAATCAGGCAAGGTCATCAGCCTGGCCTTCATGTAACCGTCGCTCC2630.13884489494245592No Hit
GTCCAGGTTCCCTTTCGGGCACAATCCCATCTCTGGAATCTTCCTGGCAT2620.13831696758526027No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCACTTCCCTACCACTTGCATG2600.13726111287086898No Hit
ACAAGCTCCCCGAAGGGTCGGTCACCTTTCGGTTTCCTACCACTTGTATG2600.13726111287086898No Hit
TCAGGCTCCCCGAAGGGTCGGTCCGCTTTCGCTTCCCTACCACCTGTATG2560.13514940344208637No Hit
GCAGCAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCACTGCAGTTC2470.13039805722732553No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCAGGATTCCTGGCAT2400.12670256572695598No Hit
CAGCAGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT2340.12353500158378207No Hit
GTGACAGCTCCTTGCGGAGAGGGTGGCTTTCACCACCTGTCTGCCCCTCT2330.12300707422658642No Hit
TTTCGTGCCTCCGAAGAGGGGGACTGATCTCTCAGCCTTTCACTCAATGT2270.11983951008341252No Hit
ATAGCACCCTCGAAGGCTGAATCCATTTCTGGAAACGTGCAGCTATAGTT2260.11931158272621688No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCC2220.11719987329743427No Hit
GCATGCTCCCCGAAGGGTCGATCCACTTTCGCTTCTCTACCACATGCATG2210.11667194594023862No Hit
ACAGGTTCCCCCGAAGGGGATCACTAACCTTTTCAGGCAGCTATACCTGC2080.10980889029669517No Hit
GCAAGCTCCCCGAAGGGTCGGTCTGCTTTCGCTTCCCTACCACTTGCATG1980.10452961672473868No Hit
GTCCAGGTTCCCTTTCGGGCACAATCTCATCTCTGAAATCTTCCTGGCAT1950.10294583465315173No Hit
AGCGACGTCCCTTGCGGGAGCCTCATGCTTTCGCAATCGTTCGTCGTTAT1930.10188997993876042No Hit
GTTCAGGTTCCCTTTCGGGCACAATCTCATCTCTGAAATCTTCCTGACAT1930.10188997993876042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT100.0017823855230.000028
CTTCGTA100.0017823855230.000028
CTTGTAG100.0017823855230.000021
GGCAGTG100.0017823855230.000023
TAGAGAC100.0017823855230.000022
AGTCAGG100.0017823855230.000026
ATAGGCT550.0230.000021
TGCCAAA100.0017823855230.000027
ATGGCGG100.0017823855230.00002230
GGACTCT100.0017823855230.000025
GACCGCT100.0017823855230.000023
GCAGTGT100.0017823855230.000024
ATAGCAC252.3483153E-9230.000021
TTCGTAA100.0017823855230.000029
TTGTAGC100.0017823855230.000022
ATAGAGA100.0017823855230.000021
TGACGTC100.0017823855230.000022
GCCATTG202.135348E-7230.000027
CCTGTAC100.0017823855230.000028
GTGGCGT252.3483153E-9230.00002230
Produced by FastQC (version 0.11.9)