FastQCFastQC Report
Wed 30 Nov 2022
4061-JW19trimB_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW19trimB_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138846
Sequences flagged as poor quality0
Sequence length231
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA5066736.49150857784884No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGGAGACTTCCTTAGTA124538.968929605462167No Hit
TAGCCGTAAACGATGGAAACTAGGTGTTGCCCGTATCGACCCGGGCAGTG95716.893248635178543No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATATTGATCAGTGTCGCA47373.4116935309623613No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGTCTGTATCGACCCGGACAGTG32652.351526151275514No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTCTTTAGTA20931.507425492992236No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGCGAGCATGCTCGTCAGTGTC18311.318727222966452No Hit
ACGCAGTAAACGATGCATACCAGGTGTGGGATGGCGTTCGCGTCGTTCCG17181.2373420912377742No Hit
ACGCTGTAAACGATGCCGGCTCGGTGCTTGGGGTCCGTTTAGGACCTTGG14931.075292050185097No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTGTCAGTGACCACGTGTCACTGAG11520.8296962101897065No Hit
ACGCCGTAAACGATGAGTGCTAGGTGCTGCGGGTATTGACCCCTGCGGTG11500.8282557653803495No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGTCATGTTGATCAGTGTCGCA11290.8131310948820997No Hit
ACGCCGTAAACGATGGATGCTAGCCGTTGGGCAGCTTGCTGTTCAGTGGC8940.6438788297826369No Hit
TAGCCGTAAACAATGGATACTAGATGTTGACCAGATCGACCTGTGCAGTA6760.4868703455627098No Hit
TAGCTGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTGCAGTA6720.4839894559439955No Hit
ACGCCCTAAACTATGGATACTCGACATACGCGATAAACTGTGTGTGTCTG6100.4393356668539245No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGTGGGAGTTGACCCCTGCCGTG5570.4011638794059606No Hit
ACGCCCTAAACTATGGATACTAGATGTTGGCCTTAGGGTCAGTGTCGCAG5260.37883698486092504No Hit
TAGCCGTAAACAATGGATACTAGATGTTGCACAGATCGACCTGTGCAGTA4810.34642697665038963No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG4610.33202252855681835No Hit
ACGCTGTAAACGATGGATACTAGGTGTGGGAGGTATCGACCCCTTCCGTG4560.3284214165334255No Hit
ACGCCGTAAACGATGAATGCTAGACGTTGGGGAGCTTAGCTCTTCAGTGT3880.2794462930152831No Hit
TAGCGGTAAACGATGGATACTAGGTGTTGTCTGTATCGACCCAGACAGTG3750.27008340175446177No Hit
ACGCCGTAAACGTTGGGCACTAGGTGTGGGTCTCAACCAACGAGATCCGC3650.26288117770767616No Hit
ACGCCGTAAACGATGAGCACTAGGTGTCGGGGTCGCAAGGCTTCGGTGCC3440.24775650720942627No Hit
ACGCCGTAAACGATGAGTACTAGGTGTTGCGGGTATTGACCCCTGCAGTG3420.24631606240006912No Hit
TAGCTGTAAACGATGGGCACTAGCTTGGGGTCTCCCTGTGTGATCCCAGG2900.20886449735678375No Hit
TGGCCGTAAACGATGGGTACTAGGTGTGGGGGGTGTCAACTCCTCCCGTG2780.200221828500641No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGAAGGAGACTTTCTTAGTA2750.19806116128660534No Hit
ACGCTGTAAACGATGAACACTTGGTGTGGAGGGAGTTGACCCCTTCCGTG2600.18725782521642684No Hit
TCGCCGTAAACGATGGATACTAGGTGTTGGGTGTGGTCAACGCATTCAGT2530.18221626838367688No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTGGTCG2530.18221626838367688No Hit
ACGCCGTAAACGATGGATGCTAGCCGTTGGGCAGCTTGCTGCTCAGTGGC2490.1793353787649626No Hit
ACGCTGTAAACGTTGTATACTAGGTGTTAGTGGCTTGTCCACTAGTGCCG2480.17861515636028405No Hit
TGGCCGTAAACGATGGATACTAGGTGTAGTGGGTATCGACCCCCACTGTG2360.16997248750414126No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG2290.16493093067139133No Hit
TAGCCGTAAACGATGGGTACTAGGTGTTGGAGGATTCTACCCCTTCAGTG2200.15844892902928423No Hit
AGGGTGTAAACGCTGCGGGCTTGGTGTTGGGGGTCCTTAGTGGGCGCCCA2150.15484781700589142No Hit
TAGCTGTAAACGATGAGTGCTGGGTGTAGGGGGTCATTACCCCCCCTGTG2110.15196692738717715No Hit
TAGCTGTAAACGATGAGTGCTGGGTGTAGGGGGTATGAACCCCCCCTGTG2100.1512467049824986No Hit
CAGCCGTAAACGATGCTCGCTAGGTGTCAGGCATGGCGCGACCGTGTCTG2060.14836581536378435No Hit
ACGCCCTAAACGATGTCTACTAGTTGTTGGTGGAGTAAAATCCATGAGTA2010.14476470334039152No Hit
ACGCTGTAAACGATGGATGCTAGATGTTGGGGCGCTTGCGTCTCAGTGTC1930.139002924102963No Hit
ACGCCGTAAACGATGGATACTAGGTGTAGGAGGTATCGACCCCTTCTGTG1880.1354018120795702No Hit
ACGCCCTAAACGATGCGAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA1870.1346815896748916No Hit
ACGCCGTAAACGATGATAACTAGCTGCCAGGGCTCTTAGAGCTTTGGTGG1860.13396136727021304No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGGGCAGTA1750.12603892081874882No Hit
CAGCTGTAAACTATGGAGACTAAGTGCTGCCGCAAGCAGTGCTGTAGCTA1730.1245984760093917No Hit
TAGCCGTAAACGATGGATACTAGGTGTTGCGCGTATCGACCCGTGCAGTA1680.12099736398599888No Hit
ACGCCCTAAACGATGTCTACTAGTTGTTGGAGGAGTGAAATCCTTCAGTA1630.11739625196260604No Hit
TAGCTGTAAACGATGTAGACTTGGCGTAGGTTGGGTAAAACCGATCTGTG1630.11739625196260604No Hit
CAGCCGTAAACGATGCGCGTTAGGTGTATCGATGACCACGAGTCATCGAG1600.11523558474857036No Hit
CAACCGTAAACGATGGATACTAAATGCTGTCGAAAACAGTGTTGTAGCTA1570.11307491753453466No Hit
TAGCCGTAAACAATGGATACTAGATGTTGAACAGATCGACCTGTTCAGTA1570.11307491753453466No Hit
ACGCTGTAAACGATGTCAACTAGCTGCTTGTGTCTTCGTGACGTGGGTAG1570.11307491753453466No Hit
TAGCCGTAAACGATACTCGCTAGGTGTCGGCCACGGTGCGACCGTTGTCG1560.1123546951298561No Hit
ACGCCCTAAACGATGCCAACTAGGTGTTGGGGAAGTAGACTTCCTTAGTA1530.11019402791582039No Hit
CAGCTGTAAACGATGCGCGTTAGGTGTATCAGTGACCACGAGTCACTGAG1500.10803336070178472No Hit
ACGCCGTAAACGATGAATACTAGATGTTGGACATATTGTTCAGTGTCGCA1500.10803336070178472No Hit
CAGCCGTAAACGATGTGCGTTAGGTGTATCGGTGACCACGAGTCACCGAG1500.10803336070178472No Hit
ACGCCCTAAACGATGTCAACTAGTTGTCGGGCCTTACTAGGCTTGGTAAC1470.10587269348774901No Hit
TAGCCGTAAACGATGGGCACTTGGTAAGGGGGATTCCTATGGTCCTCCTT1450.10443224867839188No Hit
ACGCCCTAAACGATGTCAACTAGGTGTTGGGGGAGGAGACTTCCTTAGTA1450.10443224867839188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA152.1300988E-5225.000023
ACCGATG303.092282E-11225.000029
ACCAATG152.1300988E-5225.000029
AACAATG56550.0225.09
AAACTAT2550.0225.08
TTGGCTA100.0019030496225.0225
CGGCGAT100.0019030496225.0225
CAACGAT202.3803477E-7225.08
GACCGTA252.6739144E-9225.02
TAAACCA202.3803477E-7225.07
CAACCAT100.0019030496225.01
CACTGTA252.6739144E-9225.02
GGCCCTA1950.0225.02
ACACTGT252.6739144E-9225.01
AACTATG2550.0225.09
CTGATTT252.6739144E-9225.0225
AGAGCTC100.0019030496225.0225
TAAACAA56550.0224.801067
CCGTAAA95650.0224.764774
CGTAAAC95500.0224.76445
Produced by FastQC (version 0.11.9)