FastQCFastQC Report
Wed 30 Nov 2022
4061-JW19trimB_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename4061-JW19trimB_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138846
Sequences flagged as poor quality0
Sequence length236
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT4881835.15981735159817No Hit
GTTCGCGCTCCCGAAGGCACCCTTCCCTTTCAAGAAGGTTCGCGACATGT76855.53490917995477No Hit
TACAGCTTCCCGAAGGACCTCATCTTTCAATGAGTTTAGCTGCAATATCA46473.3468735145412905No Hit
CAGCAGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT36702.643216225170333No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCGGGATTCCTGGCAT34362.4746841824755483No Hit
GTTCGCGCTCCCGAAGGCACTCCGAGCTTTCACTCAGATTCGCGACATGT29092.095126975209945No Hit
GTCCAGGCTCTCTTTCGAGCACTCCCACCTCTCAGCAGGATTCCTGGCAT28832.076401192688302No Hit
GTGGACGCCAGCCGAACTGAAGGACCCGATCTCTCGAGCCCATACGTCCC18811.3547383432003801No Hit
CAGCGGGATTCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT16281.1725220748167033No Hit
GCAGCACCCTCGAAGGAGCCGGCTTTCACCGGTTTGCAGCTACATGTCAA13860.9982282528844908No Hit
CAGCTAGTCAAGTAGAGTCTTCATCCCGACTATCATTCAGCTGTCTTATC13060.9406104605102056No Hit
CAGCAGGGTTCCGGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT12440.8959566714201346No Hit
CCGAGTGTCCTTGCGGAAAACCACTTTCATGGTCGATCACTCGAAGTTCA12360.8901948921827061No Hit
CTCCTGGCTCCTTGCGGCACGTCCATGCATCTCTGCTAGACTTCCAGGGA11710.8433804358785993No Hit
TCACTCGTTCCCCGAAAGGCACCCCGGCATCTCTGCCAGGTTCGAGTGGA10930.7872030883136714No Hit
GTTCGCGCTCCCGAAGGCACCCTTCTCTTTCAAGAAGGTTCGCGACATGT10600.7634357489592786No Hit
TACAGCTTCCCGAAGGACCTCCCCTTTCAGGGAGTTTAGCTGCAATATCA9160.6597237226855653No Hit
AGCACACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGTGCTATGTCA7670.5524105843884591No Hit
GTATACGTTCCAAACGGCACTTTCCTCTTTCAAAGAAATTCGTATCATGT6300.4537401149474958No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGCAATTCGTATCATGT6280.45229967013813865No Hit
CTGGCAGTGTATTGCTACAAAGTGAGCTTTCACCCACGGTCTCCCAGCAT5830.4198896619276032No Hit
ATACATGTCCCGAAGGAAAGACGTATCTCTACGCCGGTCATGCACAGTTC4890.3521887558878182No Hit
GTCCCGATTCCCTTTCGGGCACCCCCACCTCTCAGCAGGGTTCCGGGCAT4750.34210564222231826No Hit
ACAGCACCCTCGAAGGCGACTTCATTTCCGAAGCTTGCAGCTGTATGTCA4700.3385045301989254No Hit
GTATACGTTCCAAACGGCACTTTCTTCTTTCAAAGACATTCGTATCATGT4610.33202252855681835No Hit
GCTAGTTCCCTTTCGGACACTGACTTTCATCAGCTTTAACTAGCATGTCA4540.32698097172406837No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTATC4330.31185630122581853No Hit
GTATCCGTTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT3690.26576206732639035No Hit
CTCTGCGTTCCCTAAGGCACTTTCGAGTTTCCCCGAAATTCGCAGGATGT3540.25495873125621193No Hit
CACCTCTGCCCCGAAGGGAAGGTCCCGTTACGGACCGGTCAGAGGGATGT3230.2326318367111764No Hit
ACTACGGTTCTTGCGAAAAGGCCTATCTCTAGACCGGTCCATAGCAGTTC3120.2247093902597122No Hit
CTCCCTGCCCCGAAGGGCTTCGCAGCTTTACCTGCTATTCAGGGGATGTC3080.22182850064099793No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTAAC3050.21966783342696225No Hit
GTCCAAGCTCTCTTTCGAGCACTCCCACCTCTCAGCGGGATTCCTGGCAT2760.19878138369128387No Hit
GCAGGTCACACCCGAAGGTAATCAGCCAGCTTTCACCGGCCTAGCCCCTG2700.19446004926321248No Hit
CAGCTAGTCAAGTAGAGTCTTTAGCCCGACTATCATTCAGCTGTCTTAAC2580.18581738040706972No Hit
GTGGACGCCAGCCGAACTGAAGGACCCCATCTCTGGAGCCCATACGTCCC2360.16997248750414126No Hit
CAGCTAATCCGGCAAGGTCTTCAGCCTGGCCTTCATATCGCTGTCGGTCC2340.16853204269478417No Hit
GCAGCAGTTCTTGCGAAAAGGCACATCTCTGCACCGGTCCACTGCAGTTC2200.15844892902928423No Hit
CACTGATCCAGCCGAACTGAAGGAAAGTGTCTCCACTATCCGCGATCAGG2190.15772870662460567No Hit
TACAGCTCCCCGAAGGACCCTCCCTTTCAGGAGGTTTAGCTGCAATATCA2170.15628826181524855No Hit
ACAGCACCCTCGAAGGCGGCTTCATTTCCGAAGCTTGCAGCTGTATGTCA2140.15412759460121286No Hit
CTCCGCGCTCCCGAAGGCACCGATCCATCTCTGGAAAGTTCGCGGGATGT2140.15412759460121286No Hit
GTACGGCCCCGAAGGACACCGTATCTCTACGGCTTTTCCGTACATGTCAA2050.1476455929591058No Hit
GTATACGGTCCAAACGGCACTTTCTTCTTTCAAAGAAATTCGTATCATGT2010.14476470334039152No Hit
GTCTAAGCTCTCTCTTTCCTTTCACTTCTCCACAAGGAAGAAGAAACAAA2000.14404448093571295No Hit
GTGGGAGCCAGCCGAACTGAAGGAATCCATCTCTGGAAACCAAACTCCCC1910.13756247929360585No Hit
CTTACGGCTCCCCGAAAGGCACCCCTCGCTTTCACGAAGGTTCCGTAGAT1890.13612203448424873No Hit
GTCTAGGCTCCTTTTCAGGCACTCCCCCATTTCTGGAGAATTCCTAGCAT1860.13396136727021304No Hit
CACCTTGTCCCGAAGGACTTCCCATATCTCTATGGTATGCAAGGGATGTC1840.13252092246085592No Hit
CAGCTAGTCAAGTAGAGTCTTCAGCCCGACTATCATTCAGCTGTCTTACC1820.13108047765149877No Hit
CTTAGGGCTCCTACAAAGTAGGCACCCTCTAATTTCTTAAAGGTTCCCTA1750.12603892081874882No Hit
GTTCACTCGGGAACTTCCCCTTTCAGGGAAAAAGTGACATGTCAAGTCCT1730.1245984760093917No Hit
ACACGAGTCCCGAAGGAAGACCCAATCTCTTGGGCTGTCTCGTGCAGTTC1710.12315803120003456No Hit
CAGCGATTCAGGCAAAGTCTTCAGCTTGGCCTACATATTGCTGTCGCCCC1700.12243780879535601No Hit
CAGCAGGATCCCTGGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCAT1680.12099736398599888No Hit
GTTAGCGTTCCCGAAGGCACTAATCTATCTCTAGAAAATTCGCTACATGT1620.11667602955792748No Hit
CTCACTGTCCCCCGAAGGGGCCGACCTAATCTCTTAGGTATTCAGTGGAT1610.11595580715324894No Hit
GCAAGGGTCCCGAAGGAAGGATGTATTTCTACACCTGTCCCCTGCATGTC1520.10947380551114183No Hit
CAGCTAATCCGGCAAAGTCTTCAGCCTGGCCTTCATATTGCTGTCGGTCC1490.10731313829710615No Hit
GTCCACTTTCCCTTTCGGGCACCTAATCCATCTCTGGTTCGTTAGTGGCA1490.10731313829710615No Hit
CTCAGTGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCACTGGATGT1460.10515247108307045No Hit
GTCCCGATTCCCTTTCGGGCACCCCCGCCTCTCAGCAGGGTTCCGGGCAT1450.10443224867839188No Hit
ACAAGCTCCCCGAAGGGTCGGTCACCTTTCGGTTTCCTACCACTTGTATG1450.10443224867839188No Hit
GCACGCTGGTATTGCTACCTCGTCAGGCTTTCACCTTTCTACTACGTGCA1430.10299180386903475No Hit
CACCTCTGCTCCGAAGAGGGACTCTATCTCTAGAGTTTACAGAGGGATGT1430.10299180386903475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTG252.3446773E-9230.000021
GTCCTTG2000.0230.000027
GTCCAAG252.3446773E-9230.000021
AAAATCA252.3446773E-9230.000024
TCCGGAA151.9512774E-5230.09
AATCCGG302.5465852E-11230.06
AGTCCCG302.5465852E-11230.06
GATCGCG302.5465852E-11230.01
GTACACG151.9512774E-5230.01
CTCCGGA151.9512774E-5230.08
TCCGAAC302.5465852E-11230.09
AATTGTC151.9512774E-5230.03
GGTATTG350.0230.08
GCTCGTC151.9512774E-5230.03
AGCTAAT302.5465852E-11230.02
CAGAGTT151.9512774E-5230.03
TTACAGG151.9512774E-5230.0230
AGCAGAA151.9512774E-5230.02
ATCGCGC350.0230.02
TGGTATT350.0230.07
Produced by FastQC (version 0.11.9)